Effect Of Piper Nigrum's Active Constituent (DMF) On Proliferation And Differentiation And Expression Of Foxm1 In Ocslcs Derived From Ovarian Cancer Skov3 Cell Line

Objective: To investigate the effect of the piper nigrum's active constituent 5,7-dimethoxyflavone(DMF) on proliferation and differentiation and expression o f FOXM1 protein in ovarian cancer sphere forming cells(OCSLCs) derived from human ovarian cancer SKOV3 cell line, and explore the mechanisms underlying piper nigrum's active constituent(DMF) inhibits ovarian cancer stem cells characteristics.Methods: Human ovarian cancer was cultured in vitro. Used the suspension culture with stem cell-conditioned medium to enrich and gather the Ovarian cancer sphere-forming cells and identified as OCSLCs. The MTT assay was used to measure the effect of piper nigrum's active constituent(DMF) on proliferation of OCSLCs.The rate of pneumonosphere formation of OCSLCs was detected by pneumonosphere formation assay. And the Effect of Piper nigrum's active constituent(DMF) on its proliferation and differentiation by the cell differentiation-inducing assay. We used the Western blot to compare the expression of FOXM1 protein in SKOV3 sphere-forming cells and parental SKOV3 cells,and the effect of piper nigrum's active constituent(DMF) on expression of FOXM1 protein in OCSLCs.Results:1. The ovarian cancer sphere cells derived from human ovarian cancer SKOV3 cell line which was cultured in stem cell-conditioned medium condition growing into a sphere.2.Piper nigrum's active constituent(DMF) could inhibit proliferation of OCSLCs with the dose–dependent manner. 3.Piper nigrum's active constituent(DMF) could inhibit proliferation and differentiation of OCSLCs with the dose–dependent manner by the cell differentiation-inducing assay.4.Piper nigrum's active constituent(DMF) could inhibit expression of FOXM1 protein with the dose–dependent manner by the Western blot.Conclusion: 1.Ovarian cancer sphere-forming cells derived from ovarian cancer SKOV3 cells which was cultured in stem cell-conditioned medium have cancer stem cell characteristics. 2.Piper nigrum's active constituent(DMF) can inhibit proliferation and differentiation of OCSLCs with the dose –dependent manner. 3.The self-renewal inhibited role of piper nigrum's active constituent(DMF) of OCSLCs was associated with down-regulating expression cancer signaling molecule FOXM1. 4.Piper nigrum's active constituent(DMF) inhibited the invasion and metastasis abilities of OCSLCs.and regulated the expression level of FOXM1 protein.