The Function Of Endometrial Regenerative Cells In Treatment Of Ulcerative Colitis

Objective:To establish an experimental ulcerative colitis model in mice and explore the development and progression mechanism of UC controlled by endometrial regeneration cells.Methods:Build BALB/c UC model induced by DSS.To observe the clinical and pathological expression of laboratory mice during the molding time and estimate the model.Intervention experiment of ERC were given to three groups(normal group:given no DSS water to drink freely;untreated group:given 4%DSS to drink freely and intravenous injection PBS buffer;ERC group:given 4%DSS to drink freely and intravenous injection ERC resuspended by PBS buffer).The 7th Day,untreated group and ERC group were changed to normal drinking water.ERC were intravenous injected at 2?4?6?8 day(1×106/0.25ml/mouse)following colitis induction.Observe and record the weight.stool consistency and characteristics of bloody stools of each mouse daily during the test period,and make Disease Activity Index(DAI).All mice were killed at the 14th day,measured the colon length;test the CD3+CD8+?CD4+CD25+?CD 11c+MHC-?+ cell count of spleen by flow cytometry;determine the mRNA transcriptional level of IL-2?TNF-??IL-4?IL-10 in colon tissues use Florescent real-time quantitative RT-PCR;examine the protein expression level of Mac-1(CDllb)?MPO in the colon by immunohistochemistry.Analysis the changes of the above indexes,assess the auxo-action of ERC to the repair bowel of UC model.Results:Control DSS-induced mice developed severe colitis,characterized by body-weight loss,bloody diarrhea,mucosal ulceration and colon shortening,as well as pathologic changes of lamina propria cell infiltrations of neutrophils,macrophages,crypt distortion,lymphoplasmacytosis,and hyperplastic muscularis mucosae.ERCs attenuated colitis;Compared with untreated colitis mice,ERC reduced the number of CD3+CD8+cells in ERC group significantly(P<0.01),the number of splenic CD11c+MHC-?+ cells was significantly reduced(P<0.01),however,CD4+CD25+cells increased significantly(P<0.01);Compared with untreated group,of IL-2 and TNF-a mRNA transcription level was lower(P<0.01)in ERC group,however,IL-4 and IL-10 transcription level increased significantly(P<0.01).Between ERC group and untreated group,The protein expression level of Mac-1(CDllb),MPO in colon tissue have statistical difference(P<0.01);Conclusion 1.Successfully established a mouse model of ulcerative colitis,ERC can effectively control the occurrence and development of the experimental UC.2.ERCs play its immunosuppressive effects by inhibiting the maturation of dendritic cells,T cell proliferation and enhancing the generation of Treg cells and other mechanisms.3.ERCs may regulate the anti-inflammatory cytokines and pro-inflammatory cytokines to control UC.4.ERCs may be a promising therapeutic tool in the inhibition of UC.