SiRNA-mediated HMGA2 Gene Silencing Changes A549 Proliferation, The Expression Of Notch1 And The Clinical Analysis Of Lung Cancer

objective:As an anchitectual transcription factor,HMGA2 can regulate the transcription of a variety of genes both positively and negatively.They do not display direct transcriptional activation capacity,but regulate gene expression by binding to AT-rich regions in the DNA and or direct interaction with several transcription factors.The purpose of the study was to study the role of HMGA2 in lung cancer A549 cells’ s on proliferation and apoptosis through down-regulating HMGA2 by siRNA.Further research also played on whether the expression of HMGA2 has a relationship with the expression of Notch1,which is an important recipient in the Notch signaling pathway.To analyze the clinical features,pathological features and the roles of immunohistochemistry in lung cancers.Methods:A small interfering RNA of HMGA2 was constructed and transfected into lung cancer cell line A549 by liposome.48 hours later after the transfection,the cells morphology was observed with a phase-contrast microscope.The transfection efficiency was calculated by Flow cytometry.RT-PCR was used to detect the relative level of HMGA2 and Notch1 mRNA.Westernblot wes used to detect the expression of HMGA2 and Notch1 protein.Cell proliferation and apoptosis were determined by CCK8 and Flow cytometry,respectively.By conducting retrospective studies,,the data of 180 lung cancer patients undergoing surgery in the Department of thoracic surgery of our hospital in 2015.1~2015.6,was collected.The medical statistic analysis wes used the spss 20.0.Results:(1)The Flow cytometry showed the transfection sfficiency of A549 cells was 95%.(2)After transfection 24 hours,the shape of A549 cells changed from spindle to ellipse.And the closely intercellular adhesion was changed.(3)24 hours after transfection,the expression of HMGA2 mRNA was significantly lower than the control group.The relative level of Notch1 mRNA was also declined.(4)48 hours after transfection,the expression of HMGA2 protein was significantly lower than the control group.Westernblot analysis also shows the protein levels of Notch1 was lower than the control group.(5)Cell proliferation was significantly decreased.(6)The apoptosis was significantly enhanced in the siRNA-HMGA2 group.(7)In 180 patients with lung cancer,the proportion of adenocarcinoma was 66.7%,higher than that of squqmous cell carcinoma’s 9.4%.The proportion(51.4%)of adenocarcinoma in male patients wasgreater than squamous cell carcinoma.(8)The positive rate of P53 and EGFR in the patients with lung cancer was higher than that of the ALK.The positive rate of ALK was higher in female patients than in male patients.Conclusion:The small interfering RNA of HMGA2 can effectively inhabit the expression of HMGA2 gene in A549 cell line.Silencing of the HMGA2 gene decreased the proliferation and enhanced the apoptosis of A549 cells.Adenocarcinoma has become the most common pathological type of lung cancer and the detection of immunohistochemical index can provide clues for further diagnosis and treatment.