Detection The Klebsiella Pneumonia By The Real-time PCR Method In Sputum And Investigation Of Its ESBLs-producing Antibiotic Resistance

Objective:Compared with the bacterium culture method,to provide a rapid and accurate method that is real-time PCR for the detection of Klebsiella pneumoniae.Investigate the isolation rate of ESBLs-producting Klebsiella pneumoniae and the antibiotic resistance of Klebsiella pneumoniae isolated from our hospital(pingxiang people’s hospital).Methods:(1) The Klebsiella pneumoniae detection of 68 suspected-infection sputum samples were taken and analyzed by both bacterium culture and real-time PCR. The sensitivity and specificity of real-time PCR were also analyzed. (2) The antibiotic sensitivity testing was used to detect extend-spectrum β-lactamase(ESBLs) from strains of Klebsiella pneumoniae isolated from 169 sputum samples.Results:(1)The detection rate of Klebsiella pneumoniae by real-time PCR was significantly higher than that by bacterium culture [72.06%(49/68) vs. 48.53%(33/68), P<0.05]. The sensitivity of real-time PCR was 1×103 copies/ml and there was no cross reactions with non Klebsiella pneumoniae. (2) The ESBLs-producing Klebsiella pneumoniae was detected in 44.97%(76/169) of the isolates. The rates of resistance to antibiotics in ESBLs-producing Klebsiella pneumoniae were higher than ESBLs-negative counterparts except imipenem.Conclusion:(1) The positive rate of real-time PCR was higher than bacterium culture. The high sensitivity and specificity and the rapid turnaround time make real-time PCR valuable for effective clinical diagnosis. (2) Although clinical isolates of ESBLs-producing Klebsiella pneumoniae are still sensitive to imipenem, meropenem and pipercillin/tazobactam that can be used in the empirical antibiotics treatment, the high degrees of resistance to various antibiotic agents should be considered.