Clinical Separation And Resistance Analysis Of Klebsiella Pneumoniae

Objective: To realize the clinical characteristics and the resistance changes of Klebsiella pneumoniae in China Japan Union Hospital of Jilin University from 2013 to 2015, we did the analysis of imipenem resistant strains in order to realize the carbapenem-resistant mechanism and provide the sugesstions for the clinical prevention and control.Methods: In this study, we collected the Klebsiella pneumoniae in the submitted specimens of China Japan Union Hospital of Jilin University from 2013 to 2015. We used French bio Merieux VITEK 2 compact automatic microbial analysis system to identify the species of Klebsiella pneumonia, and also to test the drug sensitivity by the same time. Results of drug sensitivity of Klebsiella pneumoniae were analyzed by WHONET 5.6 software. Results were analyzed according to CLSI 2013-2015 break Points. The improved Hodge test and double-disk synergy test were used to identify carbapenemase. The results were analysised combined with clinical information.Results: A total of 13137 clinical isolates were detected automated from 2013 to 2015. We detected 1963 strains of Klebsiella pneumoniae, accounting for 15% of total clinical isolates. The rate of Klebsiella pneumoniae clinical isolates was 13% of 596 in 2013,15% of 684 in 2014, 18% of 683 in 2015. Neurosurgy have the higest rate of Klebsiella pneumonia in our hospital clinical department. Klebsiella pneumonia isolated from neurosurgy accounted for 16%, 21% and 20% of tatal clinical separation rate from 2013 to 2015. There were no significant changes of Klebsiella pneumoniae in the past 3 years. The rate of ESBLs-positive isolates in 2015 were higher than those isolated in 2013, accounted for 23.5%, 22.6% and 26.6%. The major mechanism of Klebsiella pneumoniae conferring resistance to carbapenem rely on the production of carbapenemase.Conlusions: 1. The rate of Klebsiella pneumoniae was the second of the clinical isolates in our hospital. 2. The first and second rate clinical department of Klebsiella pneumoniae clinical isolates were the neurosurgery and general surgery department. 3. There were no significant changes of Klebsiella pneumoniae in the past 3 years. 4. The rate of ESBLspositive isolates in 2015 were higher than those isolated in 2013. We should pay attention to this problem. 5. The major mechanism of Klebsiella pneumoniae conferring resistance to carbapenem rely on the production of carbapenemase.