Effect Of Anyiparticles On The ET?NO And TXA₂?PGI₂ Of Rats With Severe Acute Pancreatitis

Objective:To study the intervention effect of traditional Chinese medicine?TCM?Anyi particles on severe acute pancreatitis rats ET?NO and TXA₂?PGI₂.And from the level of signal transduction to explore the influence of Anyi particles for severe acute pancreatitis rats ET?NO and TXA₂?PGI₂.Methods:120 healthy male SD rats were randomly divided into 4 groups:normal control group,SAP group,IL-10 intervention group and Anyi particles treatment group,each group were 30 rats.Each group and then divided into three subgroups: 3 h group,6 h group,12 h group,each group 10 rats.Normal group in the abdominal cavity injection of saline solution?0.25 ml / 100 g?;SAP group to 6% of L Arginine?L-Arginine?solution?150 mg /100 g,1 time / 1 h,a total of three times?intraperitoneal injection preparation SAP rat model;IL-10 intervention group to give 10000 u rh IL-10?recombination human interleukin-10?after intraperitoneal injection of pretreatment,again to L-Arginine method of induction of SAP.Anyi particles group for 3 consecutive days to give Anyi pancreatic granules?4 g/Kg?aqueous solution after lavage,again to L-Arginine method of induction of SAP.After induction of SAP for each group respectively in 3 h,6 h,12 h,three time points 3 h,6 h,12 h after anesthesia group to 1% pentobarbital sodium,partial blood from the abdominal aorta,and then put to death in the rat,gather in the pancreas.By using enzyme-linked immunosorbent?ELISA?tissues of pancreatic ET?NO and TXA₂?PGI₂ The application of SPSS statistical software for Windows 17.0 will be used for statistical processing of the results.Results:?1?in addition to the normal control group,pancreatic tissues in the other groups group ET levels increased significantly in comparison with the sap group?P < 0.01?,IL-10 positive control group,an pancreatic granules group,pancreatic tissue endothelin levels significantly increased?P < 0.01?.Compared with IL-10 positive control group,there was no significant difference?P > 0.05?,3h,12 h,ET,6h?P<0.01 or P<0.05?in the pancreatic tissue of the pancreatic tissue of the pancreatic tissue of the pancreatic tissue of the pancreatic group.?2?and normal control group,pancreatic tissues in the other groups group content of nitric oxide?no?level increased significantly in comparison with the sap group?P < 0.01?,IL-10 positive control group,an pancreatic granules group,pancreatic tissue no content level significantly increased?P < 0.01?.Compared with IL-10 positive control group,there was no significant difference?P > 0.05?,3h,12 h,NO and 6h in pancreatic tissue of the pancreatic tissue of the pancreatic tissue in the pancreatic tissue of the group.?3?and normal control group,the pancreatic tissues in the other groups group ET / no ratio mean significant differences?P < 0.01?,compared with SAP group,IL-10 positive control group,an pancreatic granules group,pancreatic tissue ET / no ratio mean significant differences?P < 0.01?.Compared with IL-10 positive control group,there were significant differences in the ratio of ET/NO?P<0.01?in the pancreatic tissue of the pancreatic tissue of the pancreatic tissue.?4?and normal control group,pancreatic tissues in the other groups group TXA₂ level increased significantly in comparison with the sap group?P < 0.01?,IL-10 positive control group,an pancreatic granules group,pancreatic tissue TXA₂ level significantly increased?P < 0.01?.Compared with IL-10 positive control group,there was no significant difference?P > 0.05?,3h,12 h,TXA₂,6h?P<0.01 or P<0.05?in the pancreatic tissue of the pancreatic tissue of the pancreatic tissue of the pancreatic tissue of the pancreatic group.?5?and normal control group,pancreatic tissues in the rest of the group group of PGI₂ levels increased significantly in comparison with the sap group?P < 0.01?,IL-10 positive control group,an pancreatic granules group,in the pancreas of PGI₂ levels significantly increased?P < 0.01?.Compared with IL-10 positive control group,there was no significant difference?P > 0.05?,3h,12 h,PGI₂and 6h in pancreatic tissue of the pancreatic tissue of the pancreatic tissue in the pancreatic tissue of the group.?6?and normal control group,other groups group in pancreatic tissues of TXA₂ / PGI₂ mean significant difference?P <0.01?,compared with SAP group,IL-10 positive control group,an pancreatic granules group,pancreatic tissue TXA₂ / PGI₂ mean significant difference?P< 0.01?.Compared with IL-10 positive control group,the TXA₂/PGI₂ ratio of pancreatic tissue in the pancreatic tissue was not statistically significant?P >0.05?,and there was a significant difference in 6h?3H < 0.05?,and there was a significant difference in 12h?P<0.01??P?.?7?the pathology score of SAP group was significantly higher than that in the normal group?P<0.01?.The pathological score of the pancreatic granule group was significantly lower than that of the SAP group?P<0.05?.Conclusions:?1?Anyi particles Improve prognosis and reduce mortality on SAP rats;?2?Anyi particles can protect the SAP rat pancreatic tissue,reduce pancreatic tissue damage and improving the prognosis of SAP,protect pancreatic tissue;?3?Signaling pathway NF-?B /i NOS-COX-2 regulat ET?NO and TXA₂?PGI₂ intracellular,activation vasoactive substances is a Important hub in disturbance of microcirculation;?4?Ratio imbalance of ET/NO ? TXA₂/PGI₂ may be a important mechanism of action that cause pancreatic microcirculation disturbance;?5?Anyi particles can regulation the ratio between ET/NO and TXA₂/PGI₂,improve pancreas ischemia and hypoxia state,protect pancreas when SAP happened it explain the mechanism on microcirculationlevel.