| ?BackgroundandObjective?Bladder cancer as the most common urological malignancy in China,the histopathological types was mostly urothelial carcinoma.Bladder urothelial carcinoma can be divided into the muscle invasive bladder cancer and muscle invasive bladder cancer.Bladder cancer had a high recurrence rate,muscle invasive bladder cancer easily transfer and had a poor prognosis.The diagnosis and follow-up of patients mainly based on cystoscopy,but it is an invasive and high cost operation.Therefore,research on genetic changes in the role of the bladder cancer,explore the molecular mechanism of bladder cancer development,looking for molecular markers can distinguish between different level and stage of bladder cancer,make early detection of patients with bladder cancer and determine the risk of tumor progression,have great significance for prolonging the life of the patients with bladder cancer and improve patients life quality.With the continuous development of gene chip technology and bioinformatics analysis,a lot of molecular marker that have the value of diagnosis and prognosis of bladder cancer was been confirmed in the basic research.Cancer gene sequencing has been applied in clinical studies,and hopefully incorporated into clinical diagnosis and treatment in the future.We use the second generation of gene sequencing technology,to study the bladder cancer tissue samples,and filter out a lot of molecular markers which has differences in expression in bladder cancer.After analyzing the result of the sequencing,CENPF gene expression in bladder cancer tissues are significantly higher than normal tissue,and the expression in the muscle invasive bladder cancer is higher than in non muscle invasive bladder cancer,express quantity is stable and feasible.CENPF's main function is to control cell mitosis and adjust the cell cycle.The elevated expression of CENPF was found in a wide variety of tumor,But no related research was found in bladder cancer.?Methods?1?By the PCR and immunohistochemical experiment,validate the expression of CENPF of the 30 cases of bladder cancer patients' s tissue samples,and expression in patients with bladder cancer in different stages and stages were compared.2?Select the urine of bladder cancer patients and the control group patients,testedCENPF in urine by using the method of ELISA,comparison and analysis the clinical diagnostic value.3?Choose the T24 bladder cancer cell line,whose CENPF expression is highest in several common bladder cancer cell line.Reduce CENPF expression through transfection SiRNA,and to found the influence of CENPF on T24 cell line's functions sush as proliferation,migration,invasion,cell cycle and cell apoptosis.?Results?1 ? PCR and immunohistochemical results showed higher CENPF expression in bladder cancer tissues,expression of muscle invasive bladder cancer tissues is higher than the tissue of patients with non muscle invasive bladder cancer,the difference was statistically significant.2?The expression of the urine CENPF in bladder cancer patients is higher than the normal control group,and is associated with tumor grade and tumor stage,the difference was statistically significant.3?After CENPF is interference,T24 bladder cancer cell proliferation at a slower pace,migration and invasion ability is reduced,Cell cycle arrest at G2 / S period,and also found an increase in cell apoptosis.?Conclusions?CENPF is high expression in bladder cancer organizations,and associated with tumor malignant degree.CENPF expressed higher in the urine of the bladder cancer patients,differentially expressed in the urine of bladder cancer patients with different grading and staging.CENPF can affect the biological function of bladder cancer cells.CENPF is expected to become the diagnosis markers of bladder cancer as well as the treatment targets. |
PDF Full Text Request