| Objective: To test invastigate the meaning of Casein kinase-2(CK2)and Hematopoietic PBX-interacting protein(HPIP)and the correlationship of the two factors by testing the expressions of of the two factors in of I~V class glioma tissues defined by WHO and normal brain tissue.Methods: To collect 64 cases of glioma tissues from the second affiliated hospital of hebei medical university between from April in 2014 to October in 2015,there are 9 cases of class I,17 cases of class II,18 cases of class III,11 cases of class IV Cases of specimen were diagnosed by the pathological department of hebei university department according to the WHO diagnostic criteria for brain tumor classification.8 normal brain tissue specimens were taken from patients with traumatic brain injuries or patients with cerebral hemorrhage who needed Intracranial decompression.All the glioma specimens were diagonosed for the first times after admission and had without any anti-tumor treatment such as radiation and chemical therapy before ntracranial decompression operation.All experimental specimens were cut immediately from pathological tissue,and wered fixed by 10% formalin and were stored by liquid nitrogen at-80 ℃,All the specimens were divided into five groups: normal brain tissue,grade I glioma group,grade II glioma group,grade III glioma group,grade IV glioma group.Use immunohistochemical method to detect HPIP and CK2 expression in the pathological tissue section of the five groups,use statistical software SPSS19.0 to analyze its relevace,use immunofluorescence to detect the expressions of HPIP and CK2 and observe the distribution characteristics of the two factors.Use Western-Blot method to determine the HPIP and CK2 protein bands in the specimens of the 5 groups,and applicate related software to test grey value in the band of the five groups,use SPSS19.0 statistical analysis.Results:1 In IHC results showed that the positive rates of HPIP in normal brain tissue,I,II,III,IV grade glioma group expression were73.69±15.539%、82.79±16.246%、6.98 ± 4.604%、25.54±20.093%、53.32±23.630%,and the expression of HPIP increased with the malignant degree of tumor,Pearson rank correlation was concluded and the correlation coefficient r = 0.779,P < 0.01,the expression of HPIP was positively correlated with tumor grade,the higher tumor grade,the higher the positive rate of HPIP expression,as shown in the pictures: Fig.1、Fig.2、Fig.3、Fig.4、Fig.5,Statistical chart was shown in the Table 1、Table 2.The positive rates of CK2 in normal brain tissue,I,II,III,IV grade glioma groups were 8.97±8.379%,28.70 ± 19.848%,45.75 ± 24.881%,66.50 ± 18.170%,76.67 ±16.887%,and the expression of CK2 increased with the malignant degree of tumor,Pearson rank correlation was concluded and the correlation coefficient r = 0.712,P < 0.01,the expression of CK2 was positively correlated with tumor grade,the higher tumor grade,the higher the positive rate of CK2 expression,as were shown in the picture: Fig.6,Fig.7,Fig.8,Fig.9,Fig.10,see Table 2 、 Table 4.Correlation analysis of the expressions of HPIP and CK2 were conducted.The correlation coefficient r = 0.922,P < 0.01,the expression of HPIP was highly positively correlated with the expression of CK2,the higher level expression of HPIP,the higher level expession of CK2,as were shown in the Table 5.2 Using double immunofluorescent staining observation of two kinds of factor distribution of the same path ological tissues.The red fluore scence of HPIP factor,the green fluorescence of CK2 factor,blue fluorescent nuclear.Visible distribution of two kinds of factors in the same tissues were gathered together.Fig.11,Fig.12,Fig.13.3 Protein content: use westernblot method to determinate the CK2 and HPIP protein expessions in the specimens of the normal brain tissue,grade I,grade II,III,IV glioma groups,we use the Stripe grey value as standard.Analyse the relative expression of HPIP and CK2,the higher relative expression of HPIP,the lower relative expression of CK2.All levels of glioma group had significant differences from that of normal brain tissue in the expressions of the two factors,using the single factor analysis of variance.Results was shown by mean + /-standard deviation,says P < 0.05,As were shown in the Table 6,Fig.14,Fig.15,Fig.16.Conclusion:1 CK2 and HPIP protein contents in glioma tissues were higher than those of nomal tissues,the higher tumor grade,the higher level of CK2 and HPIP protein contents,and had significant differences between the groups.CK2 and HPIP protein contents in glioma tissues were higher than those of normal tissues,the higher tumor grade,the higher level of CK2 and HPIP protein contents,the malignant level can affect the CK2 protein expression to a certain extent.2 It can Can be seen in the immunofluorescence images that in multiple sets of specimens,the CK2 protein expression site and HPIP protein expression is coincidence.Combined with statistical analysis,CK2 and HPIP has certain relevance.3 Through the normal brain tissue and different grade glioma group immunohistochemistry analysis,Spearman and Pearson rank correlation between P<0.01,we could infer that in a large extent HPIP expression were positively correlated with CK2 expression. |
