In Vitro Inhibition Of Plasma On Candida Albicans

Objective To study the influence of cold atmospheric plasma on the growth of albicans Candida, and to explore the possibility of treating patients with Candida albicans infection. It will provide a new way of thinking and treatment for clinical treatment of Candida albicans infection.Methods ATCC10231 was selected as the experimental strain. The standard Candida albicans was cultured for three times, then selected the appropriate amount of Candida albicans into sterile ground, dilution, and finally the concentration of two groups of Candida albicans was 1 x 108 and 1 x 107CFU/ml, respectively.100ml of these two groups were evenly coated on the sand Fort agar plate for 30 minutes. In order to guarantee the accuracy and the randomness of the experimental results, the two concentrations of each group were randomly selected from each of the 15 test plates, and 3 experimental groups were set up under the same experimental conditions. Under the condition of argon and oxygen reaction environment, medium barrier was discharged (dielectric barrier discharge method, DBD) to produce cold atmospheric plasma, and by cold atmospheric plasma on Petri dishes for different times of irradiation. After irradiation the medium flat plate placed at 37℃ constant temperature incubator culture, Candida albicans culture colony growth was observed in the culture medium for 3 consecutive days. In order to determine the irradiation of cold atmospheric plasma on Sabouraud’s culture dish on Candida albicans growth effect, two concentration groups and different irradiation times of the experimental groups were those differences.Results By cold atmospheric plasma were irradiated for 0,30,60,120 and 180 seconds after the subjects Sabouraud’s culture dish. When placed in a constant temperature of 37℃ for incubator for 24 hours, the uniform growth of colonies of Candida albicans Culture Center at the irradiation position, there were obvious bacteriostatic ring, with the extension of exposure time, bacteriostatic annulus increases gradually. After continuous observation for 3 days, all experimental groups Petri dish of bacteriostatic ring size did not changed. The antimicrobial ring size was negatively correlated with the concentration of Candida albicans. The concentration of Candida albicans was lower, the antibacterial ring was bigger than that of higher concentration on the flat plate in the same cold atmospheric plasma irradiation time. The antimicrobial ring size was positively correlated with the cold atmospheric plasma irradiation time. The cold atmospheric plasma irradiation time was longer, the antibacterial ring was greater on the flat plate in the same concentration of Candida albicans.Conclusion The cold atmospheric plasma can inhibit the growth of Candida albicans in vitro. It can be maintained for a long time, which may be a new, safe, economical and reliable method for the treatment of Candida albicans infection in the future.