Discrimination Analysis Between The Human Skin Normal Cells And Cancer Cells Based On ECIS

Skin cancer, with a rising trend year by year, attracts widespread attention. Particularly, the squamous cell carcinoma, which can migrate to other tissues of the body, has a higher mortality rate. This paper takes the squamous cancer cells A431 and normal skin cells HaCaT as the research object, compare the difference between A431 and HaCaT, hoping to achieve early detection of the skin cancer.ECIS, in the form of on-line and non-destructive, can be able to analysis adherent cells morphology and behavior. Compared to other methods, ECIS has the following advantages:high sensitivity, even can detect the early small changes; high throughput, can monitoring cell activities continuously, has access to dynamic information; no label, reduce the use of expensive reagents; non-invasive, the ECIS determination process has no hurt to cells:high degree of automation, data can be automatically generated and collected. Based on these advantages, this paper chose ECIS as detection method. Experimental results showed that the ECIS detection device in this paper provided a simple and real-time method to tell the distinction between the cells. We hoped that those results can pave the way for a label-free, non-invasive identification of the two cells. In all. this paper consists of the following four chapters.Chapter 1 PrefaceIn this chapter, we firstly introduced cancer, cells and cancer cells. Then kinds of methods to detect the skin cancer cells in the literature at home and abroad were listed, including biopsy, optical detection, and the tumor markers. In this chapter, emphasis was put on the introduction of ECIS, including its principle, development, as well as the application in drug testing and cells distinguishing. And following is the research purpose, significance and the innovation of this paper.Chapter 2 Design and fabrication of ECIS deviceIn order to improve the stability of impedance detection devices, we designed a new ECIS system to measure cell impedance. This device combined cell culture with impedance detection in a small plate, which was more reliable, convenient and easy to operate. In this small chamber, continuous cell cultivating, in situ photo catching and real-time electrical determination, all can be achieved. In this chapter, we specifically introduced the design, fabrication and use of this device. The whole device was depicted by CAD drawing,3D model was built with SolidWorks. To our knowledge, this device can reduce the variance of electrical impedance and enhance the sensitivity.Chapter 3 Monitoring and analyzing growth behavior of normal skin cells by using ECISECIS can monitor the cell status change, when cells attach on electrode, cell number and morphology changed over time, the corresponding impedance will change as well. This chapter used ECIS to monitor the growth behavior of HaCaT, compared with the picture counting method, the results arrived in a same conclusion. We used the impedance analysis software Zsimpwin. fitted the data by using the equivalent circuit. HaCaT cell parameters were obtained. Thus ECIS provides a new platform for observing cell growth behavior.Chapter 4 Discrimination between the human skin normal cells and cancer cells by using ECISECIS is a biological method to measure impedance signal caused by the change of cells. It has become a novel tool to identify cancer cell without any biomarkers. In this chapter, ECIS was used to distinguish skin cancer cells A431 from normal skin cells HaCaT. Firstly, impedance data were recorded, then, equivalent circuits were used to fit the impedance result, this two cell types have different parameters value, which can be a criterion for distinguishing. In this way, ECIS provides an easy and real-time monitoring tool to study cells difference, it does not require any additional reagent neither any pretreatment of the sensor. We expect that in the future such a device can replace the conventional methods for cells differentiation.