The Correlation Study Of Vitamin D Receptor Gene FokⅠpolymorphism And Dyslipidemia

Objective: A case-control study to analyze the characteristics of the VDR gene FokⅠ single nucleotide polymorphism genotype frequency and allele frequency in normal population and dyslipidemia populations.To research the relationship between the distribution of VDR gene FokⅠ polymorphism and dyslipidemia.Methods:(1)In this paper,the research objects were Han population in Sichuan Luzhou area whose age is between 40 to 80 years,and among individuals without genetic relationship.According to the epidemiological information and clinical laboratory test results,the people were divided into two groups: normal control group of 304 cases,male 95 cases,female 209 cases;dyslipidemia group,301 cases,male 96 cases,female 205 cases.(2)genomic DNA were extracted from blood samples.VDR gene FokⅠ polymorphism genotypes were detected by restriction fragment length polymorphism-polymerase chain reaction(RFLP-PCR)method.To verify the accuracy of defined genotype by DNA sequencing and to calculate the frequency of genotype and allele.(3)Statistical analysis: all the data were analyzed with the IBM SPSS19.0 Statistical software package.The distribution balances of genotype were verified by Hardy-Weinberg equilibrium law.The difference of distribution of genotype and allele were detected by chi square test.The dyslipidemia disease risk was analyzed by the two variables logistic regression.Results:(1)Compared two groups of clinical data show that,compared with the control group,the TC,TG,LDL-C,BMI and WC were increased in hyperlipidemia group,and HDL-C level were lower than the control group(P<0.001).(2)The distribution of genotype show that the VDR gene FokⅠ polymorphismin population were in accord with the genetic equilibrium by Hardy-Weinberg equilibrium law test(P>0.05).(3)Compared with the reference database of NCBI gene polymorphism data,the distribution of VDR gene FokⅠ polymorphism in this study is similar to the Asian population,but different with Europe and Africa american populations.(4)genotype frequencies of ff,Ff,FF were 20.6% 、 47.5% 、 31.9% in lipid abnormalities group and 22.4%、54.9%、22.7% in control group respectively.The difference of genotype in normal control group and dyslipidemia group was statistically significant.Compared with Ff/ff genotype,showed an increased risk to develop lipid abnormalities(OR=1.595,95%CI=1.111-2.290,p=0.011).Allele frequency differences were not significant(P>0.05).(5)Analyzed the genotype difference between dyslipidemia subtype,compared with the control group,the genotype difference and genotype frequency distribution in hypercholesterolemia was not significant.The difference of genotype in normal control group and hypertriglyceridemia group was statistically significant(P=0.041).Compared with Ff/ff genotype,showed an increased risk to develop hypertriglyceridemia(OR=1.784,95%CI:1.134-2.267,P=0.012).Allele frequency differences were not significant(P>0.05).(6)Comparison of clinical datas and genotypes of all study groups,TG levels increased significantly in all population with FFgenotype(P<0.05).(7)Multiariable Logistic regression analysis,the result shows that FokⅠ polymorphism、BMI are lipid metabolic disorder independent risk factors.Conclusion:(1)The differences of genotype distribution were significantly between dyslipidemia group and normal control group.There is a correlation between dyslipidemia and VDR gene FokⅠ polymorphism.(2)There is a associated between VDR gene FokⅠ polymorphism and hypertriglyceridemia,FF genotype could be a risk factor of hypertriglyceridemia.(3)Comparison of clinical datas and genotypes of all study groups,TG levels increased significantly in all population with FF genotype.(4)FokⅠ gene polymorphism、BMI are lipid metabolic disorder independent risk factors.