The Correlation Research Between Vitamin D And Its Receptor Gene Polymorphism And Metabolic Syndromein Yunnan Han Population

Objective:To investigate the relationship between Vitamin D and metabolic syndrome components (waist circumference, BMI, blood pressure,glucose). As well as the relationship between VDR gene including loci FokI (rs2228570)、BsmI (rs1544410)、ApaI(rs7975232)、TaqI (rs731236) polymorphism and metabolic syndrome. In order to provided some relevant data and theoretical basis for improving the epidemiological data and study of the metabolic syndrome treatment targets.Methods:(1) Collected the clinical data and biochemical indicators in 180 patients in Yunnan with type 2 diabetes who attend the department of DM in first affiliated hospital of Kunming medical university region from November 2013 to August 2013.Among the cases, there are 81 cases with type 2 diabetes and the metabolic syndrome,99 cases of type 2 diabetes patients without metabolic syndrome) and 96 control cases. (2)QPCR-taqman probe method was used to detect the genotype and allele frequency of FokI, BsmI, Apal, TaqI as vitamin D receptor genetic locus in above objects.(3 Statistical methods to analyze:SPSS21.0 statistical system was used to compare the biochemical indicators and genetic data by the statistical analysis and logistic regression analysis. To Compare the difference of 25 (OH)D3 concentrations between the three groups, analysis the relationship between 25 (OH)D3 levels and metabolic syndrome components (waist circumference, BMI, blood pressure, blood sugar),detect the frequency of vitamin D receptor genotypes and allele, and analyzes its relationship with the susceptibility of the metabolic syndrome.Results:(1) There are statistical differences in BMI, age,25 (OH) D3, FPG, glycosylated hemoglobin, fasting C peptide, SBP, DBP, TG, HDL-C, waist circumference between MS and NC groups(P values< 0.01). Plasma 25 (OH) D3 concentration of NC group was significantly higher than that in DM group and MS group.(P< 0.01). (2) 25(OH)D3 is negatively related to the TC(P< 0.01). (3) ff、Ff、FF、bb、Bb、BB、tt、Tt、 TT、aa、Aa and AA distribution difference has no statistical significance between MS group and DM group, MS and NC group, DM and NC group (P> 0.05).Logistic regression analysis results suggest that Age, BMI, TG, SBP, waist circumference may be the risk factors of MS(P=0.043,0.048,0.043,0.048,0.000, OR=1.119,1.368, 3.457,1.159,1.221),and Fasting plasma C-peptide,25 (OH) D3, DBP and HDL-C may be the protective factor of MS (P= 0.005,0.046,0.005,0.046, OR=0.147,0.832, 0.815,0.003). (4) Logistic regression analysis results suggest that genotype TT (P= 0.001, OR=18.600) may be the risk factors of metabolic syndrome, and genotype BB may be the protective factors of metabolic syndrome (P=0.000, OR=0.088). Linkage disequilibrium analysis results suggest that linkage disequilibrium may exist in BsmI and TaqI gene locus. (D’=0.782).Conclusion:Plasma 25 (OH) D3 concentration in patients with Metabolic syndrome was significantly lower than normal people; age, BMI, TG, SBP might be the risk factors of metabolic syndrome. Fasting plasma C-peptide,25 (OH) D3, DBP, HDL-C may be protective factors of metabolic syndrome; genotype TT might be the risk factors of metabolic syndrome, genotype BB may be protective factors of metabolic syndrome; linkage disequilibrium may exist in BsmI and TaqI gene locus in MS.