Based On 3D Mechanical Environment Of Myocardial Stem Cells Engineered Micro- Myocardial Tissue Construction Reasearch

Heart diseases such as myocardial infarction and congestive heart failure have been associated with a high mortality rate, which is accompanied by a large number of cardiac cell death and decreased cardiac function. Other symptoms, such as heart disease, arrhythmia,cardiac drug screening, needs an ideal organizational model in vitro to understand cell transplantation in myocardial function after improvement and reduction of myocardial hypertrophy and the infulence of cell transplantation on stem cell differentiation, proliferation and differentiation of cultured cardiac fibroblasts of the impact or effectiveness of the drug.Cell therapy is the most widely used in cardiac repair, which is designed to improve the heart function or repair the damaged myocardium. There are many types of cells that can be used in cardiac repair, and they all have a certain effect, but these methods are flawed, such as cell survival,differentiation, and cross-linking in the host body. The emergence of cardiac tissue engineering,to a large extent, solved the problem of cell therapy. This method aims to construct the engineered myocardial tissue of the damaged myocardium by using cells, bio-materials and related growth factors. Thus, use of cardiac stem cells, myocardial cells and cardiac fibroblasts as seed cells to develop a model of myocardial tissue in vitro, for the improvement of myocardial damage in cell transplantation therapies, to know seed cells in tissue mechanical environment on proliferation and differentiation, drugs interfere with the heart's function provides the possibility of in vitro models.Stem Cells Cardiac(CSCs) has the characteristics of adult stem cells, and autologous transplantation will not occur immune rejection, therefore, cardiac stem cells are the ideal cell source for the construction of engineered cardiac tissue. Matrigel contains a variety of ECM proteins and growth factors, can serve as ideal material for building 3D cardiac tissue engineering. This paper explores in the mechanical context, to building 3D engineered cardiac tissue in vitro. Matrigel and DMEM medium proportion according to different dilution, cardiac stem cells and myocardial cells and cardiac fibroblasts cells with Matrigel mixed in certain liquid.Using FlexCell mechanical loading equipment, firstly the six holes of tissue culture forminggrooves,sceondly the mixed cell is added into the Grooves, for rest for 40 minutes, 3D construction, and then rest for 2-3 days, and then use the FlexCell tensile loading mechanical loading equipment, construction of 3D engineered cardiac tissue in vitro.With the right mechanical environment, cells ratio and dilution ratio of 3D gel, build a 3D micro-tissue, developed in vitro 3D tissue method to construct the myocardial tissues for diseases such as myocardial injury in an in vitro study, lay the organizational models that are available.This paper is divided into four chapters. Chapter one is the introduction, comprehensive introduction to the myocardial infarction and the current the main treatment; Chapter two working for cell isolation, purification, culture and identification; Chapter three use Flexcell mechanical loading system, constructing 3D engineered myocardial tissue, and detection of myocardial cells of micro myocardial tissue; The fourth chapter is the summary of the whole thesis and work prospects.