Study On Construction Of Engineered Cardiac Tissue

Objectives: To construct engineered cardiac tissue (ECT) using liquid collagen as scaffold based on cardiac myocytes as seed cells derived from newborn rats and embryonic stem (ES) cells, respectively.Contents: The effects of cell seeded density and collagen quantity on constructionof ECT based on liquid collagen as scaffold are investigated. Construction of ECTbased on cardiac myocytes derived from ES cells is established.Methods: We isolate cardiac myocytes from ventricles of newborn rats digested bytrypsin and set up methods obtaining mass cardiac myocytes from ES cells based onmass production of EBs using rotary cell culture system (RCCS), induction of EScells into cardiac myocytes using ascorbic and enrichment of cardiac myocytes usingPercoll discontinuous gradient centrifugation. Based on cardiac myocytes derivedfrom newborn rat ventricles and ES cell, respectively, ECT is constructed using liquidcollagen as scaffold.Results: Methods of isolation, culture and identification of cardiac myocytes fromnewborn rat ventricles and mass cardiac myocytes obtaining from ES cells areestablished. ECT characteristic of native muscle is constructed based on cardiacmyocytes derived from newborn rat ventricles and ES cells.Conclusions: ECT characteristic of native muscle can be constructed based oncardiac myocytes derived from newborn rat ventricles and ES cells.