Effect Of SGK1 In Premature Aging Placenta

Objective:In the study, we investigated the expression of SGK1 in normal and premature aging placenta, and the effect of hypoxia on the fibrosis of HTR-8/SV neo cells, and SGK1 inhibitor on HTR-8/SVneo cells fibrosis, meanwhile, to explore the treatment of premature aging of placenta.Methods:(1) Pregnant women received prenatal examinations and delivered at The Second Affiliated Hospital of Chongqing Medical University during the period of December 2012 to November 2013. Twenty cases with premature aging placenta diagnosed by ultrasonography were enrolled. The control group comprised 20 gestational-matched normal pregnant women. The expression level of SGK1 and CTGF in placenta was detected by immunohistochemistry, western blotting and real-time RT-PCR. The fibrosis extent of placenta was detected by Masson's tri chrome staining. (2) We cultured HTR-8/SVneo cells in vitro. Cells were divided into three treatment groups:a normoxic conditions group (21% O2?5% CO2), a hypoxic conditions group(5% CO2?97% N2), and a hypoxic+SGK1 inhibitor group. Cells were cultured for 48 h.The expression level of SGK1 and CTGF in each group was detected by western blotting and real-time RT-PCR. Cells were cultured for 72h. The fibrosis extent of each group was detected by Masson's trichrome staining.Results:(1) SGK1 was noted mainly in the membrane and cytoplasm of placental trophoblast cell. Western blotting and real-time RT-PCR revealed that premature aging placenta had higher expression of SGK1 compared with normal placenta. Masson staining revealed that normal placenta tissue existed fibrosis, and that, the extent of fibrosis was increased in premature aging placenta. Meanwhile, the expression level of CTCF in premature aging placenta was increased.(2) In vitro, Hypoxia increased SGK1 and CTCF transcription in HTR-8/SVneo cells. Adding SGK1 inhibitor (GSK650394) in HTR-8/SVneo cells cultured in hypoxic conditions, decreased the expression of SGK1 and CTCF.(3) In vitro, Hypoxia induced the production of more collagen fibers, Adding SGK1 inhibitor (GSK650394) in HTR-8/SVneo cells cultured in hypoxic conditions, decreased the production of collagen fibers.Conclusions:(1) The expression level of SGK1 in premature aging of placenta was significantly increased. Meanwhile, The expression level of CTGF in premature aging of placenta was also significantly increased. It is suggest that SGK1 may play an important role in the pathogenesis of premature aging of placenta.(2) Hypoxia can induce the expression of SGK1 and CTGF increasing in HTR-8/SVneo cells. Hypoxic plays a promoting role in the fibrosis of HTR-8/SVneo cells.(3) SGK1 inhibitor decreased the expression level of SGK1 and CTGF in hypoxic group. SGK1 inhibitor can reduce the fibrosis extent of HTR-8/SVneo cells.