| Objective: To Study the neuroprotective effect of rhEPO on premature rats with intrauterine infection WMD through detection of the changes in MOG and EPOR in brain tissue and assessment of neuroethology.Method: Selected 12 wistar rats that had been pregnant for 15 d and divided them into infection group(n=9) and control group(n=3) by ratio 3:1 at random, conducted intraperitoneal injection of LPS(0.3mg/Kg) and equivalent stroke-physiological saline solution on 15 th day's pregnancy of Wistar rats in both groups respectively and conducted cesarean section to take out fetal rats on 21 th day's pregnancy; observed pathological changes by taking out fetal rats(n=6) and new rat brain tissue(n=10) hematoxylin eosin(HE) staining in two groups respectively; randomly selected 32 newborn rats in the control group as the black control group; selected 64 newborn rats in the infection group and randomly divided into EPO treatment group(n=32) and infection control group(n=32). Regarding EPO treatment group, conducted intraperitoneal injection of rhEPO(5000IU/Kg) promptly every day after the birth. Regarding black control group and infection control group, conducted intraperitoneal injection of equivalent saline solution simultaneously. As for newborn rats in three groups, took 8 premature rats on 0h,3rd,7rd and 14 th day of their birth respectively and took out brain by perfusing formaldehyde; detected the level of MOGmRNA by RT-PCR,detected the level of MOG prot ein by western blot and EPOR in tissue through elisa; as for these three groups.Result: 1. The mortality of pregnant rats after injection of LPS was 18.2%, and the delivery days of the pregnant rats was 19.0-20.5, the rate of prematur e delivery was 100%. The delivery days of the control group was 22.0-22.5 days.2. Intrauterine LPS inoculation produced inflammation of placenta that featured congestion of blood vessel and edema with a lot of visible neutrophile granulocytes infiltrated,but all cases n of control group had no histologic evidence of intrauterine infection.HE staining procedures revealed clear staining and normal structure of periventricular white matter from the black control group. Weak staining and focal rarefaction of periventricular white matter were shown in the infection group; the thickness of ne rve fiber was uneven with disorder in move. It could be seen that there were deeply stained and few protuberant less-differe tiated nerve cells. The number of glial cells was increased. It could be seen that there were changes in apoptosis such as karyopyk nosis, loose cytoplasm and cell shrinkage.3.Compared with 0 hour group, the level of MOGmRNA and EPOR is higher than that in 3 day?7 day and14 day of blank group. The difference has statistical significance(P<0.05). MOGmRNA of brain tissue in blank control group obviously decreased than that in 3da y and 7day infection control group. But the level of EPOR significantly increased and the difference has statistical significance(P<0.05). Compared with infection control group, the level of MOGmRNA and EPOR is higher than that in 3 day ?7 day and14 day of EPO treatment group. The difference has statistical significance(P <0.05). At the same time, level of MOG protein detected by Western blot in EPO treatment group is significantly higher than that in the infection control group with the same age in 3 day?7 day and 14 day group.(P<0.05)Conclusion:1.By intraperitoneal inflammation of LPS in pregnant rats that can lead to increased incidence of preterm pregnant rats;And it cause damage to the brain tissue of premature rats.2. Intrauterine inflammation can inhibit the expression of MOG gene in brain tissue, but can induce endogenous EPOR rise.3.EPO shows a protective effect to neonatal premature neonate brain injury after intrauterine infection by promoting the myelin gene and MOG protein development and remodel myelin.4.EPO can up-regulate the MOG gene and protein and increase expression of EPOR protein, we can conclude EPOR plays an important role in the EPO protective effects of nerve repair.5.EPO is superior to be administered at early time than at later t ime. And different courses of EPO show different effect on myelin gene and protei. The 14 day course of treatment is currently the best course of treatment. |
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