Depletion Of Histone Demethylase KDM5B Inhibits Cell Proliferation Of Hepatocellular Carcinoma By Regulation Of P15 And P27

Background: KDM5B,a jmjc domain-containing histone demethylase,plays a central role in regulating chromatin dynamics and transcription,and it is reported to be up-regulated in a variety of human cancers such as bladder cancer,lung cancer,colorectal cancer,prostate cancer and malignant melanoma.In the present study,we try to detect the expression and investigate the role of KDM5B in hepatocellular carcinoma.Methods: We evaluated KDM5B expression in 50 paired HCC specimens using real time PCR and western blot.We determined its correlation with clinico-pathological parameters of HCC patients.Besides,we assessed the effect of KDM5B depletion on HCC cell proliferation both in vitro and in vivo.Results: KDM5B expression was frequently up-regulated in human hepatocellular carcinoma(HCC),and its expression was positively correlated with HCC clinical parameters.Depletion of KDM5B expression significantly inhibited HCC cell growth both in vitro and in vivo.Furthermore,KDM5B depletion leads to transcriptional activation of p15 and p27 and regulates HCC cell growth in a p15-and p27-dependent manner.Conclusion: Depletion of KDM5B could suppressed HCC progression and raises the possibility that KDM5B is a potential new therapeutic target for HCC treatment.