| The sustainable utilization of resources is an effective way to save energy. At present, the researches of the traditional Chinese medicine resources have mostly focused on the entire environment, rarely concentrated in the single medicinal resource. Therefore, this study investigated the sustainable utilization of the Japanese ampelopsis by analyzing the chemical composition of Ampelopsis n-butanol part, TLC identifying, and determining the content in the quality standard.Most of previous researchers extracted compounds from Japanese ampelopsis by using the small and medium polarity petroleum ether and ethyl acetate, however, few studies examined the extraction by n-butanol instead. This study analyzed the chemical composition on the Ampelopsis n-butanol part, purified and finally structural determined nine compounds, bb-1:4-Hydroxyphenethylalcohol, bb-2:L-Epicatechin, bb-3:Gallicacid, bb-4:Ethylgallate, bb-5:Aromadendrin, bb-6:Catechin, bb-7:(-)-Gallocatechin, bb-8:Coumaric acid, and bb-9:(-)-Epicatechin-3-gallate respectively.The optimal extraction parameters of gallic acid content determination conditions in Japanese ampelopsis were as follows:the concentration of methanol was 50%(v/v), the ratio of sample to methanol was 1:10, and the time of ultrasonic extraction was 60min. This study used Dikma Diamonsil C18 (250 x 4.6 mm,5μm) chromatographic column,0.2% acetonitrile/phosphoric acid (3:97) as mobile phase in degrees of elution, flow rate of 1.0 ml·min-1, column temperature of 30℃, detection wavelength of 270 nm to determine the content. The results provided the technical references for the sustainable utilization of resources to develop the Ampelopsis and improve the quality standard. |
