Expression Of Gene P66Shc MRNA On Peripheral Blood Monocytes In Patients With Coronary Heart Disease And Its Relationship With Oxidative Stress

Objective:To investigate the expression of P66Shc mRNA on peripheral blood monocytes(PBMs) in patients with coronary heart disease(CHD) and its relationships with oxidative stress.Methods:According to clinical manifestations and results of coronary angiography, sixty seven patients in the Department of Cardiology were recruited to this study from December2012to June2013.They were divided into three groups:normal control group (Normal, n=23), stable angina pectoris group(SAP,n=23) and acute myocardial infarction group(AMI,n=21).The levels of serum superoxide dismutase (SOD) and malondialdehyde (MDA) activity were examined to analyze the state of oxidative stress state in all subjects. PBMs were isolated and the expression of P66Shc mRNA was determined by quantitative real time PCR.Results:The levels of MDA and P66Shc mRNA were greatly increased and SOD were significantly decreased in AMI group in comparison with SAP and Normal groups. There was no significant difference between SAP group and Normal group on the levels of serum SOD,MDA and P66Shc mRNA (P>0.05). The expression of gene P66Shc was in positive correlated with the level of MDA(r=0.49,P=0.024), but not with the level of SOD in AMI group.Conclusion:This study suggests an involvement of P66Shc in the transition of a stable angina pectoris to an acute myocardial infarction. P66Shc was associated with states of increased oxidative stress. P66Shc mRNA levels were increased in AMI patients, which may be involved in the mechanism of transition from a stable to an unstable lesion.