| Objective:To study the influence of sevoflurane on the expressions of the hippocampus growth-associated protein43 (GAP-43) and neural cell adhesion molecule (NCAM), and the cognitive function of neonatal rat. Methods:In the experiment, there were 45 SD rats aged 7 days with no confinement of the male and the female while their weights were limited from 15g to 20g. The rats were divided into 5 groups (n=9 each) by the random number table method:groups L1 and L2 inhaled 1.5% sevoflurane in oxygen for 6 h and 2 h respectively;groups H1 and H2 inhaled 3% sevoflurane in oxygen for 6 h and 2 h respectively;group C inhaled 30% oxygen only. After 7 days of anesthesia, Morris water maze training for 7 days was started, measuring the navigation and space exploration ability. After the test, the rat hippocampus was obtained upon the decollation. The Western blot method was then applied to detect the expressions of hippocampus GAP-43 and NCAM. Results:1. Compared with the group C,the mean escape latency for the immature rats in L2, H1 and H2 group was prolonged. The residence time of platform quadrant and the times to run across the platform was decreased. GAP-43 expressions in nerve cell of newborn rats’hippocampus were decreased (P<0.05). There was no statistically significance for group L1 and control group (P>0.05).2.There was no statistically significance for NCAM expression of rat hippocampus in each group (P>0.05). Conclusion:Sevoflurane anesthesia had the influence on the cognitive function for neonatal rats, which might be involved with the decreasing expression level of hippocampus GAP-43. There were no effects for sevoflurane anesthesia on NCAM expression of neonatal rats’hippocampus. |
