Green Soluble Powder Compound Board Role In Prevention And Cure Of Infectious Bronchitis Research

Infectious bronchitis?IB?is an acute,high contact transmitted diseases caused by Infectious bronchitis virus?IBV?.The disease popular in farms all over the word,brings poultry industry huge economic losses.At present,due to the high intensityand wide range of use of IBV vaccine,variation of IBV virulence and antigenicity,it is hard to have good immune effect of vaccination.sometimes even induce the disease to occur.In recent years,traditional Chinese medicine in the prevention and treatment of virus disease such as IB research gradually deepen.And traditional Chinese medicine?TCM?with wide application range,low toxicity and drug residues,the advantages of economical and practical,Is widely used in livestock and poultry disease control and prevention.This test on the basis of TCM syndrome differentiation analysis of the IB,with clear lung antipyretic,cough expectorant for prescription principle,select radix isatidis,radix astragali,folium isatidis,radix scutellariae and other 9 kinds of Chinese medicinal materials,to make into a compound board green soluble powder,and AA chicken as the target animal for prevention and control of infectious bronchitis effect research.This research is divided into three parts: 1 IBV-M41 strain virulence determination and the build of IB chicks model.With consecutive rejuvenation 3 generation of IBV-M41 vaccination infection chicken embryos,observed within 7 days of deaths and survival of chicken embryos and calculate the IBV-EID₅₀ M41;According to IBV-M41 virulence determination results,Respectively apply different concentration of 10⁰EID₅₀,10¹EID₅₀,10² EID₅₀,10³EID₅₀ of IBV-M41 into the age-of-15-days Chicks without immunity by intranasal infection.According to the incidence and mortality of chicks,select the best model of toxic dose.Test result: the measured IBV-M41 EID₅₀ to 10^-4.32/0.1m L;Measured 10² EID₅₀ group the incidence of 10⁰%,and mortality rate of 0,10² EID₅₀ attack is the best drug dose.2 Compound board green soluble powder for artificial infection IB chicks treatment dose of screening.Will 10⁵ chicks be divided into 7 groups,respectively,5 observed group?H1,H2,H3,H4,H5?and model control group and healthy controls.With 10² EID₅₀ not immune attack toxic dose of 15 age chicken poison?with the exception of the health control group?,with different concentration compound board green soluble powder treatment,by observation of symptoms,determination of production performance,screening of dosage.Test results: the treatment groups the varying degree to improve the production performance,the chicks H2 group cure rate up to 86.67%;The Treatment groups are improved in different degrees of chicks thymus index,bursa of fabricius index.According to the results of the screening he determination of dosage is 6g/kg/d.3 Compound board green soluble powder,for the prevention and treatment effect of the study of the IB.150 chickens were randomly divided into 10 groups,respectively,prevention of high,medium and low dose group,high,medium and low dose treatment groups,Shuang Huang Lian control,Ma Xing Shi Gan control group,model control group and healthy controls.Prevention group starting from the age of 12 days,continuous water for 3d.In addition to the healthy controls,the rest each chicken in the age of 15 days,intranasal canister,0.2 m L.Treatment group starting from the age of 16 days,continuous water dosing 6 d.During the test record of each group of feed intake,weight gain,chicks and cure only number,calculate the material weight ratio,incidence,protection and cure rate;And examine each chicken immune organ index,lymphocyte proliferation,cytokine levels in serum,green soluble powder compound board of IBV infection prevention and cure effect of chicks.The results show that the compound board green soluble powder to prevent high dose group?8 g/kg/d?which can reach 66.67%,treatment dose group?6 g/kg/d?the cure rate is 83.33%,the control effect is remarkable.Found in each target detection,compound board green soluble powder can obviously improve the production performance of IBV-M41 infected chicks;Improve the immune organ index of infected chicks;Enhance lymphocyte proliferation ability;In addition,the compound board green soluble powder can also adjust the serum IL-2,IL-4,TNF-?,IFN-? level,keep the dynamic balance.Comprehensive indicators,compound board green soluble powder has better prevention and treatment effect,nd the treatment effect is better than the that of prevention.Of which preventation high dose group?8 g/kg/d?and the treatment dose group?6 g/kg/d?is the most effective.