The Effect Of Honeysuckble Compound Preparation On The Immune Function In Immune Organ Of Chickens Infected With NDV

Newcastle Disease is an acute and highly contagious disease caused by Newcastle disease virus which is one of the main loemia and harmful to the poultry industry. Now, the specific remedy for anti-NDV have not been found, useing chinese herbal to prevent and cure Viral infectious disease of chickens has very widest prospect. Honeysuckble compound preparation was made of Honeysuckble, Fructus Forsythiae, Herba Houttuyniae, Radix Astragali, Rhizoma Atractylodis Macrocephalae, Radix Glycytthizae that were screened by the laboratory. Honeysuckble compound preparation had the action to improve the specificity and unspecificity immunity function of the chickens'peripheral blood had been proved by Another portion test of the topic. Based on these studies, 21-days-old chickens were used in this study, and also technology of cell culture, MTT chromometry, immunoenzyme histochemical method, radio- immunifaction were used to study Fully and systemic on the effect of honeysuckble compound preparation on the T lymphocytes and subpopulation group, B lymphocytes in immune organ (thymus, spleen, bursa of Fabricius)of chickens infected with NDV; The effect of honeysuckble compound preparation on the lymphocytes proliferative responses in immune organ (thymus, spleen, bursa of Fabricius) of chickens infected with NDV; The effect of honeysuckble compound preparation on the number of(IgG,IgM)antibody-producing cells in immune organ (bursa of Fabricius, spleen, thymus) of chickens infected with NDV and the effect of honeysuckble compound preparation on splenetic T lymphocytes cytokine (IFN-γand IL-2)of chickens infected with NDV. Intend to reveal the effect and mechanism of honeysuckble compound preparation on the cellular immunity and humoral immunity in immune organ of chickens infected with NDV. The results were as follows:The percentages of CD3+, CD4+T lymphocytes and the ratios of CD4+ and CD8+ T lymphocytes in thymus and spleen of chickens infected with NDV were raised by honeysuckble compound preparation; The percentages of B lymphocytes in spleen and bursa of Fabricius of chickens infected with NDVwere raised by honeysuckble; T lymphocytes proliferative responses in thymus and spleen and B lymphocytes proliferative responses in spleen and bursa of Fabricius of chickens infected with NDV were increased by honeysuckble; The number of(IgG,IgM) antibody-producing cells in (bursa of Fabricius, spleen, thymus) were increased by honeysuckble; The honeysuckble compound preparation could promote the secretion of splenetic T lymphocytes (IFN-γand IL-2), which indicated that cellular immunity and humoral immunity of chickens strengthened by honeysuckble compound preparation and indicated indirectly that honeysuckble compound preparation had determinate action on resisting NDV.