Coexpression Analyze Of Rcie Polycomb Complex OsFIE2-PRC2 And Functional Analysis Of Polycomb Gene OsEMF2b

The function and composition of PRC2 were conserved between animals and plants.It regulate phase transition of plant through H3K27me3 and repress target genes expression.OsEMF2b is component of rice PRC2 complex,homolog to Arabidopsis EMF2.Reports of EMF2 and OsEMF2b mainly about reproductive stage.Our lab has observed that homozygous mutant of OsEMF2b or down regulation of OsEMF2b had obvious defeats in vegetative growth and seed development,our study focus on the function of OsEMF2b in vegetative stage and seed development,meanwhile,we discussed the function of OsFIE2-PRC2 through co-expression analyze.The main results are as follows:1.The expression pattern of OsFIE2-PRC2 members:OsFIE2,OsEMF2b,OsCLF and OsSET1 were similar and composition expressed.It suggested that OsFIE2-PRC2 have important function in the whole development process of rice.The expression level of OsCLF in reproduction organs were much higher than in vegetative organs,indicated that OsCLF play a key role in reproduction stage.2.Co-expression analyze of OsFIE2-PRC2 showed that OsFIE2-PRC2 have a close relationship with epigenetic regulation;co-expression network analyze of OsCLF suggested that OsCLF closely related with cell cycle and DNA metabolic.MOC1 was co-expression with OsEMF1b,OsFIE2 and OsSETl,we supposed that PRC2 regulated plant height and tillering through MOC1.3.Phenotype analyze of OsEMF2b-RNAi plants showed that the dwarf of OsEMF2b-RNAi plants were appeared at seedling.The elongation of internode is later and shorter than wild type.More than half of OsEMF2b-RNAi plants lack the near root internode.4.Section analyze of OsEMF2b-RNAi developing seeds showed that down regulated of OsEMF1b result in pre-cellization endosperm and precious embryo.Prepare for the pre-germination of OsEMF2b-RNAi seeds.5.We screen the yeast two hybrid library through the VEFS domain of OsEMF2b,found 6 candidate interacting proteins.One of them qLTG3-1 have been reported and had function in response to cold during seed germination.Combined with the co-expression result and precious reports of EMF2,we supposed that OsEMF2b participate in cold response through qLTG3-1.