Effects Of Monoammonium Glycyrrhiza On Fat Deposition In Fatty Liver Cells Of Dairy Cows And Its Regulatory Effect

Dairy Fatty Liver is one of the perinatal nutrition metabolic diseases.And decreased dairy cows milk production,induced abomasum and other diseases.The cost of treatment is higher.It affects the healthy development of China's dairy farming industry.In our study,we would established the model of fatty liver cells in primary dairy cows with sodium oleate,and research the effects of Monoammonium glycyrrhizinate(MAG)on fatty liver cell model.1.Selection of modeling conditions Using different concentrations of sodium oleate(0,0.1,0.25,0.5,1 m Mol / L)on the original culture of calf liver cells 12 h found that when the concentration of sodium oleate was 0.25 m Mol / L,hepatocyte injury could occur.Oil-red O staining showed that the fatty liver cells were labeled as ring-shaped cells.Observed by transmission electron microscopy,the mitochondrial vacuolization in the model group was serious.The best modeling concentration is 0.25 m Mol/L.And then 0.25 m Mol/L sodium oleate to induced different times(0,3,6,9,12,24 h).It was found that hepatocyte injury and oil red O staining The presence of fatty liver cells in the symbolic ring cells,to determine the 0.25 m Mol / L sodium oleate in the induction of dairy cow liver cells for 9 h in the case of as an in vitro dairy cow fatty liver cell model of the best conditions.2.MAG on the cell model in vitro Using the 0.25 mg/ L of MAG on the primary liver cells pretreatment for 12 h,and then use the best conditions to induce modeling and found that MAG can improve liver cell activity,reduce liver damage,reduce cells Lipid deposition,down-regulation of lipid-related genes Ch REBP,lipid metabolism key gene PPAR?,lipid transport-related gene MTP.These indicate that MAG can alleviate lipid deposition in cell models,reduce lipid metabolism in key genes to delay intracellular lipid peroxidation,reduce the functional burden of mitochondria in major oxidative sites,thereby reducing the degree of cell damage to protect liver cells The3.Effects of MAG on Blood Lipid and Liver Lipid Deposition in Different Layers of MAG(0,25,50,100,200 mg/ L)as drinking water for 21 days after drinking,foundthat serum TG content compared with the control group(P <0.05),but the decline trend did not change with the concentration of dosing,indicating that MAG can improve the blood triglyceride(Triglyceride,TG)levels.Serum Glucose(GLU),total cholesterol(CHOL)were not significantly different from those in control group.The serum ALT levels in the control group decreased significantly,indicating that MAG can play a protective role in the liver and reduce liver cell damage.In summary,the use of sodium oleate to establish in vitro model of the best conditions for 0.25 m Mol / L sodium oleate induced 9 h,MAG in vitro hepatocytes with delayed cell damage,reduce lipid deposition,MAG can improve the blood TG level,protect the liver,reduce liver cell damage,MAG in the production of dairy cattle to provide a theoretical basis.