The Isolation And Identification Of Ectomycorrhizal Strains In The Rhizoshere Of Chimonobambusa Utilis

Chimonobambusa utilis is one of the Chinese unique bamboo species,and the bamboo shoots are born in the autumn not spring,as well as delicious and highly nutritional.Because of its special habitat,narrow distribution and poor reproductive capacity,the Chimonobambusa utilis are really in short supply.Mycorrhization is a technology of symbiosis between fungus and plant,play the role of promoting growth and keeping health of plant.Mycorrhization has been gradually showing outstanding contributions in the agro forestry ecosystem.So we try to inoculate the fungus to the Chimonobambusa utilis and aim to deal with problems in planting.However,there is little report on the study on the mycorrhization of Chimonobambusa utilis,and obtaining the fungus and the technology of inoculation become the key of the new research.In order to obtain the Ectomycorrhizal strains,we investigated the resources of the timberland of Chimonobambusa utilis in Shixi Town of Tongzi Country,Huanglian Town of Tongzi Country and the timberland of mixed Fargesia spathacea and Cyclobalanopsis glauca in the Fanjing Mountain Nature Reserve of Jiangkou Country in Guizhou Province,systematic investigation and collection were conducted.And these strains were identified and isolated in the laboratory,then we screened the culture condition and determined of enzyme activity of some strains.The main results are as follows:1.The results showed that 24 specimens of wild macromycetes were obtained in Shixi Town of Tongzi County and 19 species were identified,include 8 species Ectomycorrhizal strains,35 specimens of wild macromycetes were obtained in the Fanjing Mountain Nature Reserve of Jiangkou Country and 22 species were identified,include 20 species Ectomycorrhizal strains,and 14 specimens of wild macromycetes were obtained in Huanglian Town of Tongzi Country and 9 species were identified,include 5 species Ectomycorrhizal strains.On the whole,73 specimens of wild macromycetes were obtained,and 45 species wereidentified by classification,include 30 species Ectomycorrhizal strains.30 species Ectomycorrhizal strains belong to 10 Russulaceae,5 Amanitaceae,6 Tricholomataceae,3Boletaceae,3 Hygrophoraceae,2 Clavulinaceae and 1 Sclerodermataceae.2.The study has identified these collected strains by combining morphology with phylogenetic analysis,and obtained 5 pure Ectomycorrhizal strains(T1 and T2: Lactarius vividus,T20 and T28: Megacollybia marginata)and other 4 strains.3.The study has screened the different carbon source,nitrogen source,temperature and pH of pure Ectomycorrhizal strains,and specially optimized the liquid culture of T2,because of its fast growth and high energy.The results showed that the optimum growth temperature is25 ℃,the optimum pH is 5,the optimal carbon sources is glucose,the optimum nitrogen source is the Hydrolyzed milk protein and the cost-optimal volume of liquid is 20ml/50 ml of T2,as well as T2 should be let stand to cultivate because it is aerobic.But the optimum speed is 100r/min if we need to product the hypha ball by rotating manufacture.As for T20 and T28,they are the same specie but come from different specimens,and there are some discrepancies on the individual,but they are consistent as the Megacollybia marginata.For T20 and T28,the optimum growth temperature is 25 ℃,the optimal carbon sources glucose,the optimal carbon sources is soluble starch,the optimum nitrogen source is the Hydrolyzed milk protein,but the optimum pH is 6 for T28,and T20 grown well in the pH of 5 or 6.The Ectomycorrhizal strains of Chimonobambusa utilis is reported firstly.This paper has investigated the Ectomycorrhizal strains resources in the Chimonobambusa utilis and identified by classification,the results showed that there are abundant Ectomycorrhizal strains in the rhizoshere of Chimonobambusa utilis.And the paper has obtained 5 pure Ectomycorrhizal strains and studied the influence of different culture conditions for the growth of strains,then studied their characteristics of enzyme activity.Above all,this study has obtained pure Ectomycorrhizal strains in the Rhizoshere of Chimonobambusa utilis and cultivated them.