Effects Of Different Resistance Rootstocks On Growth And Resveratrol Content Of 'Cabernet Sauvignon'

?Object?In this experiment,the varieties of wine grape ‘Cabernet Sauvignon' grafted on different rootstocks were used as experimental materials.The objective of this experiment was compare the concentration of Resveratrol and its precursors and the activity of important metabolic enzymes in ‘Cabernet Sauvignon' grape with eight different rootstocks suitable for Xinjiang Province.The relationship was analyzed between the rootstocks and Resveratrol synthesis.The results provide a reference for selecting resistant grape rootstocks.?Methods?The eight rootstock cultivars(Fercal,5C,140 R,3309M,3309 C,SO4,Kangzhen3,5BB)were grafted with scions of ‘Cabernet Sauvignon' grapes.Two-year-old,self-rooted‘Cabernet Sauvignon' plants were used as the control(CK).Mature leaves were collected from the scions and analyzed to determine shoot length,rootstock diameter,scion diameter,chlorophyll content and other growth indicators and photosynthetic characteristics(Pn?Tr?Ci?Gs).The content of trans-Resveratrol,trans-piceid and phenylalanine,cinnamic acid,and coumaric acid were determined by HPLC techniques.The activities of the following enzymes in the phenylalanine pathway of Resveratrol production were also determined:peroxidase(POD),polyphenol oxidase(PPO),phenylalanine ammonia lyase(PAL),cinnamic acid-4-hydroxylase(C4H),4-coumarate coenzyme A ligase(4CL),Malonyl-coenzyme A(Malonyl-CoA)and 4-coumaric acyl coenzyme A(4CA).?Results?(1)‘Cabernet Sauvignon' grapes and rootstocks were planted in the open field.The results showed that the resistance rootstocks had inhibited on growth of biennial ‘Cabernet Sauvignon' grape.The effect of different rootstocks on the growth was significantly different.Resistant rootstocks could significantly improve the chlorophyll content of the‘Cabernet Sauvignon' grape.Only CS/Kangzhen3 combination the emergence of 'little feet'phenomenon,and more serious.CK,CS/3309 M and CS/3309 C,the soluble sugar content of CS/3309 C was better than that of other treatments.The rootstock thickness,scion thickness and chlorophyll content of CS/3309 C were also larger than those of other panicle,but the leaf area was smaller.The content of chlorophyll CS/Kangzhen3,leaf area and scion roughness larger;CS/Fercal thickness rootstock and scion diameter were higher than other treatments;the resistant rootstock could significantly increase the chlorophyll content of scion varieties of grape leaves.(2)The photosynthetic rate and water use efficiency of CS/Fercal combination were significantly higher than those of other rootstock combinations,and there was nosignificant difference with the self-rooted scions.CS/140 R transpiration rate and stomatal conductance were significantly higher than the control and other treatments;CS/Kangzhen3 transpiration rate and intercellular carbon dioxide concentration was significantly lower than the control and other treatment;CS/SO4 photosynthetic index were lower than other treatment.(3)Leaf Resveratrol concentrations were greater in all the grafted scions than in the self-rooted one.Therefore,these resistant rootstocks can significantly increase the Resveratrol concentrations in the leaves of wine grapes.Among the resistant rootstocks in this study,leaf Resveratrol concentrations were highest in scions with 140 R rootstock.Scions with 140 R rootstock also had the highest phenylalanine and coumaric acid concentrations.Leaf cinnamic acid concentrations were greatest in scions grafted on3309 M rootstock.The enzyme C4 H was the key to Resveratrol synthesis in grape leaves.In addition,PPO also had significant influence.?Conclusion?The tested rootstock had an inhibitory effect on various indicators of ‘Cabernet Sauvignon' grape.The results showed that the resistance rootstocks had inhibited on growth of biennial ‘Cabernet Sauvignon' grape.The effect of different rootstocks on photosynthetic characteristics was significantly different.Resistant rootstocks could significantly improve the chlorophyll content of the ‘Cabernet Sauvignon' grape.CS/140 R was the most advantageous combination for increasing Resveratrol in ‘Cabernet Sauvignon' grape leaves.CS/3309 C,CS/Fercal,CS/Kangzhen3 and CS/140 R had a good effect on the growth of ‘Cabernet Sauvignon' grape.