The genetic differentiation & diffusion and control of Xinjiang rice water weevil Lissorhoptrus oryzophilus(RWW)were studied in this article,this research illustrated the invasion pathway and diffusion capacity of RWW and Proposed techniques which meet the criteria of green rice production,in addition,in order to ascertain dominant species of natural enemies and the useage of Chemical pesticide was decreased to minimum,the structure of arthropod communities in different modes was studied,which will be providing a basis for the effective control of the RWW & Rice production safety in xinjiang.The conclusions was following:1、Analysis on genetic diversity of the RWW based on RAPD markers: the genetic characterastics of RWW which collcected from Northern rice production of seven geographic populations by RAPD markers.It showed that the genetic diversity of the XJYLQapqal populations is the lowest(PPB: 46.76%,H: 0.2106,I: 0.3239),while HLJAC is the highest(PPB: 62.58 %,H: 0.3006,I: 0.4450),UPGMA clustering results show that the geographic population’s between HBTS City and JLGZL city cluster prior,and then formed HLJAC,YL-Qapqal county and HC county in Xinjiang as the first clade,while the other populations were XJWJQ and XJMD.It indicated that there may be the difference of RWW source in Xinjiang.The populations of RWW in XJYLQapqal and XJYLHC probably originated from HeiLongJiang rice planting areas in China,While the XJWJQ and XJMD originated from others rice planting areas,From a chronological perspective,The populations of RWW in XinJiang probably originated from the same rice planting areas in China.2、Population and Diffusion Ability of Overwintering RWW: Occurrence of RWW in the ILI Valley,The source of RWW mainly from the Ridge at early field and the population of RWW gradually declined as the distance from the field edge increased in rice fields.its began to migrate in early May,with migration reaching a peak in late May.while RWW in the late-transplanted areas came mainly from the early-transplanted areas.Adult populations in the late-transplanted areas reached a peak immediately after the rice was transplanted and then declined gradually until almost no insects were left in early June.In general,90% of overwintering adults were located within 40 m from the field edge before transplanting.This indicates that the diffusion distance of RWW is limited and their diffusion ability is weak.3、Green prevention and control technology of RWW: the rice yield loss rate and ET of RWW was1.1756%,5.82,respectively.In aspect of pestcides screening,there was significant difference amount oxicity of different insecticides to adult(p<0.01),the lethal effect of others pesticide were well beside 4% berberine SA,8 billion spores per gram Metarhizium anisopliaeDP,6% Bacillus polymyxa AS.The field efficacy show that only 0.6% Matrine AS was 30.81% which ineffective with controlling.While others insecticides showed better control effect,The field spray control of 10 billion spores/mL Beauveria bassiana OD、1.5% Pyrethrins AS、7.5% Rotenone EC and 6% Spinetoram SCwere 51.24%~82.55% after 15 d.10 billion spores/mL Beauveria bassiana OD、1.5% pyrethrins AS、7.5% rotenone EC and 6% spinetoram AS are good for popularization and can be effective means of RWW in the green rice production.The study of Eppo uav field spray showed that The field spray control of thiamethoxam was 93.43% after 14 d.others were 80.96%~93.27%,besides,using thespecialty auxiliaries could improve effect between 22.29% and 28.49%.4、Technology of integrated control: The prevention and control of RWW should follow the principle of strengthening quarantine,scientific monitoring,precision control.With the premise of a scientific and accurate sampling monitoring and forecasting,the quarantine on non-epidemic areas should be strengthened.While the epidemic areas is given priority to harm monitoring and population inhibition.On the basis of this,economic threshold for this pest insect should be estimated.Meanwhile,many other coordinated comprehensive technologies,such as environmental friendly chemical control and biological control & physical control and ecological regulation were taken to minimize the dose and the number of pesticide,creating an environment beneficial for its natural enemy to reproduction and transfer.All these measures could promote the virtuous circle of the paddy field ecosystem for the prevention and control of rice water weevils sustainably. |