QTL Analysis Of Panicle Length,Grain Length And Width Of Specific Germplasm In A Rice

Excavation,innovation and utilization of genetic resources with independent intellectual property rights has a very important practical significance.Panicle and grain traits are important rice yield components.In this study,we cultivated a new rice germplasm named LPBG08 with semi-dwarf(65cm),long spike(46cm),large grains(70g)as materials,constructed a population BC1F2 through backcross with Nipponbare.Built the long panicle-short panicle and large grain-little grain DNA pool,and then used the technology of BSA and SSR to initially map the panicle length,grain length and grain width QTLs.It provided a scientific basis for further mapping,separation of the major gene and cloning.Preliminary mapping results showed that one grain width QTLs(qGBl)were detected on chromosome 1,two spike length QTLs(qPL3-1,and qPL3-2)and 1 grain length QTL(qGL3)were detected on chromosome 3.qPL3-1 is located in the range of RM6329-RM3-2.it is a major gene,mainly expressed as additive effect,the contribution of its additive effect to the phenotypic variation was 17.4%;qPL3-2 is located in the interval of RM15861-RM15932,a major gene,mainly expressed as overdominant(dominant degree:2.29),the contribution of the dominant effect to the phenotypic variation was 4.56%,the dominant direction is the negative;qGL3 is located in the interval of RM3856-RM6329,a major gene,mainly expressed as additive effect,the contribution of additive effect to the phenotypic variation was 18.8%;qGB1 located on chromosome 1,RM259-RM575 interval,is a major gene,mainly expressed as complete dominance(dominance degree:1.13),the contribution rate of dominant effect to the phenotypic variance was 7.37%,the dominant direction is efficiency.