The Effects Of CPPU And Different Fruit Bags On Aroma Component And Biosynthetic Genes Expression In 'Shine Muscat' Grapes

This dissertation is to investigate the difference in the aroma of the different varieties of grapes,and the effects of GA3,CPPU and bagging on aroma component and biosynthetic genes expression in grape.The main results were as follows:1.There were significantly difference in the aroma of the different varieties of grapes.Fatty acid metabolism is the main metabolic pathways for the synthesis of aromatic compounds.C6 compounds were the main aromatic compounds by the fatty acid metabolism.There were no significantly difference for the synthesis of aromatic compounds by the amino acid pathway.Terpenoids metabolic pathway was important for the roses scent grapes to produce characteristic aromatic compounds.The main characteristic aromatic compounds of rose sent grapes 'Shine Muscat'were linalool,geraniol,cis-geraniol,alpha-terpinenol,cis-linaloloxide.The main characteristic aromatic compounds of the non-roses scent grapes'Summer Black' were ethyl acetate,(E)-2-hexenal,(E,E)-2,4-hexadienal,hexanal,hexanol.The main high levels of aromatic compounds of the non-fragrant scent 'grapes 'Manicure Finger' were(E)-2-hexenal,hexanal,ethyl acetate.2.Compared with the control,The CPPU treatment decreased the species of aromatic compositions,reduced the terpenoids relative content,such as Linalool,cis-Linaloloxide,Geraniol,and weakened the flavor.The high CPPU concentration was not conducive to the generation of aroma.;Compared with the control,the peaks of the expression of biosynthetic genes of DXS3,GPPS,LIS were delayed and down-regulated,and they were delayed longer and down-regulated lower with the increasing CPPU concentration.The gene expressions were suppressed.3.The fruits with transparent bags increased the species of aromatic compositions,promoted the terpenoids relative content,such as Linalool,cis-Linaloloxide,Geraniol,and strengthened the flavor.The relative content of terpenodis in fruit with black bags were reduced,which weakened the flavor.The species of aromatic compositions in fruits with green bags were the least.The flavor was appropriate in fruits with white bags.The expression of biosynthetic genes,DXS3,GPPS,LIS were up-regulated,which was beneficial to promote monoterpenes generated.The peaks of the expression of biosynthetic genes of DXS3,GPPS,LIS were delayed and down-regulated,which was not beneficial to generate monoterpenes.The expression of fruits with green and black bags were moderate.