Effect Of Bacillus Subtilis QM3 On The Seeding Of Wheat Under NaCl Stress

Salt stress affected the normal plant growth and development,serious and even lead to plant death.Which will greatly reduce the crop yield,so increasing the salt tolerance of plants has become a necessary research project for sustainable development.At present,bioremediation technology has been preferred,its pollution-free,side effects.Bacillus subtilis is a highly resistant antagonistic strain that is rich in soil and microbial communities and contributes both to plants inthe soil and protects plants from saity contamination.In this study,some morphogical and physiological and biochemical indexes of B.subtilis QM3 under different concentrations of wheat were studied by using wheat as experimental material.The aim of this study was to provide some scientific basis for alleviation of plant salt damage.The main conclusions are as follows:(1)NaCl stress could inhibit the biomass and plant morphology of wheat.The NaCl concentration of medium and high concentration(100-300 mmol/L)decreased with the increase of NaCl concentration,and the NaCl stress of low concentration(50 mmol/L)increased the biomass and morphological indexes of wheat These indicators of wheat have played an inhibitory effect.The application of different concentrations of B.subtilis QM3 could significantly relieve the NaCl stress at low(50-200 mmol/L)concentration,which increased the above indexes and increased the salt concentration.(2)NaCl stress significantly inhibited the root activity of wheat and increased the relative permeability of the cell membrane.The activity of wheat roots increased with the increase of NaCl concentration,and the activity of cell membrane increased rapidly with the increase of NaCl concentration.The root activity of wheat was significantly increased(p <0.05)and the cell membrane permeability was decreased(p<0.05)after applying B.subtilis QM3.(3)NaCl stress increased the content of soluble sugar,proline and MDA in wheat seedling.The content of soluble protein increased gradually under low concentration(50-150 mmol/L)under NaCl stress.At high concentration(200-300 mmol/L)under NaCl stress.Different concentrations of B.subtilis QM3 significantly increased the protein content of soluble sugar and high salt stress,and decreased the contents of MDA and proline(p<0.05).(4)NaCl stress inhibited leaf photosynthetic system.The chlorophyll content,Fv/Fm,Fv/F0 and qP of wheat leaves decreased with the increase of NaCl concentration under the medium and high concentration of NaCl stress,while the low concentration of NaCl treatment could promote the photosynthetic system of wheat leaves under 50 mmol/L low concentration(p<0.05),q N and Y were opposite.Under the low concentration of NaCl stress,the application of B.subtilis QM3 could significantly increase the above values(p<0.05),but under the high NaCl concentration,the inhibition was inhibited,and the above values were decreased,the phenomenon of the above-mentioned values decrease.(5)Induction of SOD Activity increased in Wheat by NaCl stress,the activity of POD,CAT and APX increased first and then decreased with the increase of NaCl concentration(p<0.05),B.subtilis QM3 solution could significantly increase the SOD activity of wheat under NaCl stress;The activities of POD,CAT and APX in wheat under low NaCl stress were not significantly increased(p>0.05),under high NaCl stress were significantly increased(p<0.05).The activity of SOD,POD,CAT and APX in the roots of wheat increased(p<0.05)in the environment of bacteria without NaCl stress.In addition,wheat was not subjected to NaCl stress,and B.subtilis QM3 was used to increase the growth and metabolism.