The Physiological Difference Comparison Of The NaCl And Na₂CO₃Stress On Festuca Arundinacea

Tall fescue (Festuca arundinacea L.), also known as tall fescue, is the Gramineae fescue is a perennial herb. Native to Europe, in China’s Xinjiang, Central Northeast wet areas are distributed with the turf industry in China in recent years, the rapid development of tall fescue because of its root system, the ability of solid earth; wide adaptability, persistence, anti-prominent disease, drought, heat, shade, easy to form turf and other characteristics, are widely used in various types of playground, turf farms, gardens, green parks, and slope green and vertical greening, and even soil erosion-prone areas and other places turf establishment. But more serious in some area of land salinization, to some extent, which greatly limits the scope of application of the tall fescue and floor space effect.Order to understand the salt and alkali tolerance of tall fescue, thereby improving the adverse physiological traits of tall fescue, so as to provide reference for soil improvement, breeding technology research, and further promote China’s agriculture, urban greening development. This study, tall fescue seedlings grown for7days, respectively, at a concentration of50～250mmol/L of a neutral salt (NaCl) and a concentration of25～125mmol/L of basic salts (Na2CO3) Stress. For7days, respectively, using tetrazolium (NBT) method, colorimetry, spectrophotometry, Coomassie Brilliant Blue G-250staining, phenol method for the determination of the water content, soluble protein, soluble sugar, chlorophyll content and SOD, POD activity and other physiological and biochemical indicators of NaCl and Na2CO3on the physiological and biochemical indicators of tall fescue. Experimental results show that:1. NaCl and Na2CO3stress on tall fescue leaf water content50mmol/L Na+concentration of NaCl and Na2CO3water content in plant is the same, with the increase of the concentration of both salt stress, water content in tall fescue leaves gradually reduced, and Na2CO3on the water content in plant affect NaCl big.2. NaCl and of Na2CO3stress on tall fescue leaf soluble protein contentWith the increase of the concentration of NaCl and Na2CO3Stress, soluble protein content curve line type, is to increase the overall trend. In50mmol/L Na+concentration, the NaCl soluble protein is greater than Na2CO3. Subsequently, in the same Na+concentration, soluble protein content is of NaCl below Na2CO3.3. NaCl and of Na2CO3stress on tall fescue leaf soluble sugar contentWith the increase of the concentration of NaCl and Na2CO3Stress, tall fescue content of soluble sugar overall trend is increasing. Under NaCl stress on soluble sugar was a line type, under Na2CO3stress, soluble sugar content increased with increasing concentration. In250mmol/L Na+concentration, NaCl stress, the rapid increase in soluble sugar content, Na2CO3stress on soluble sugar content decreased rapidly. Of impact larger than NaCl.4. NaCl and of Na2CO3stress on tall fescue leaf chlorophyll contentNaCl and Na2CO3stress, the chlorophyll content increased with increasing Na+concentration in the leaves of tall fescue. In the same concentration of Na+,Na2CO3chlorophyll content of NaCl on chlorophyll content. In250mmol/LNa+concentration, both relatively close.5. NaCl and Na2CO3Stress on Leaves of superoxide dismutase SOD activityNaCl and Na2CO3Stress, the overall trend of SOD activity is first rose and then dropped. In under50mmol/L NaCl stress, SOD activity was significantly decreased, and subsequently increased with the increase of the concentration of stress. In stress150mmol/L NaCl, SOD activity to fall. In stress25mmol/L Na2CO3, SOD activity increased slightly, and then all the way down with the increase in the concentration of Na2CO3stress. The experimental results show that:Na2CO3on SOD Activity of SOD activity than NaCl and Na2CO3on tall fescue injury more serious than NaCl.6. NaCl and Na2CO3stress on tall fescue leaf peroxidase activity of PODPOD activity gradually increased with the increasing concentration of NaCl and Na2CO3. POD activity increased slowly in a low concentration of NaCl stress. Subsequently with increasing concentration increased significantly. With the increase of the concentration of Na2CO3processing, POD activity curve line type. In50mmol/L Na+concentration, Na2CO3processing of POD activity and NaCl treatment very close. Then with increasing concentration increased significantly in150mmol/LNa+stress, Na2CO3processing of POD activity has declined, but still higher than the activity of NaCl treatment. Experimental results show that:in the same concentration of Na+Na2CO3POD activity than NaCl. Alkaline salt Na2CO3POD activity of tall fescue in the neutral salt NaCl, also shows that the damage to tall fescue.In summary, with increasing salt concentration, the water content of tall fescue seedlings decreased SOD live a downward trend, but in a few concentration indicators increased. Seedling soluble protein, soluble sugar, chlorophyll content and POD activity increased gradually with increasing salt concentration. The same concentration of Na,50mmol/L Na+concentration, two salt water content, soluble protein, soluble sugar and POD activity. With its concentration increased performance, most of the alkaline salt on the physiological and biochemical indicators of tall fescue than the neutral salt. Therefore, the tall fescue neutral salt tolerance stronger than alkaline salt.