| This research is about that with 3 varieties of Kalanchoe blossfeldiana:'Loren'?'Fiesta'?'Theron',which imported plants have no roots as the test material.Primarily,breeding the three imported and no roots plants to three generations by cottage.At the seeding stage,flower bud stage,and full-bloom stage,we measured this 10 fancy points about:plant height,stem diameter,canopy,pitch number,internode length,flower twig number,flower number,pedicel length,petal number,corolla diameter and it's root activity respectively.Then this paper analyzed the pertinence of the development in underground part with the degeneration by cuttage.Furthermore,this paper explored the rejuvenation technology by the somatic embryogenesis and induce approach.Comparing the rejuvenated plants and degenerate plants about 10 fancy points above,which can provide the theoretic support for production of Kalanchoe blossfeldiana in our country and lay the foundation for high quality plants of Kalanchoe blossfeldiana production with standardization and large scale.The main research results as follows:(1)The fancy character of this 3 varieties:plant height is 15-16cm in average,stem diameter is about 15.5±1.5mm,canopy is about 14-15cm,pedicel length is about 4±1cm,and the flower twig number,flower number,corolla diameter have some different between this 3 varieties.The degeneration has appeared after productting one generation,and the difference was not obviously at seeding stage and was obviously at full-bloom stage.The degree of degeneration is 'Fiesta'>'Loren'>'Theron'.(2)The studying of rejuvenation technology shows that:with the leaf as the explant in somatic embryogenesis can get tissue culture seeding which is healthy and strong,and have good fancy point in there growing process.In the tissue culture process,the tissue culture seeding will be malformation easy with the stem as explant,besides,the tissue culture seeding will be thin and delicate and the inductive rate will be small with the petal as explant.With the leaf as the explant,and MS ± 6-BA1.0mg/L ±NAA0.1mg/L as the medium,and joining in some sucrose and agar powder,the 'Loren' and 'Fiesta'will be induced embryonic callus in abundance,and the inductive rate is 85.01%,82.44%respectively.The inductive rate of 'Theron' will get 68.41%with the MS ± 6-BA2.0mg/L ± NAA0.2mg/L as the medium and joining in some sucrose and agar powder.In the process of somatic embryogenesis shows that the concentration of AC joined have some impact for it's induction.In the process from embryo to little plants,the concentration of 6-BA joined is different in there best medium of this 3 varieties.In the process of enrichment culture shows that the appreciation coefficient is increased as the concentration of 6-BA(0-0.4mg/L)increase.(3)Comparing the rejuvenated plants which has been cutting propagation for six generations and the imported plants which has been cutting propagation for three generations shows that;there is no difference between the rejuvenated plants which has been cutting propagation before three generations with the imported plants about the 10 fancy points.There is no significant difference between the first,the second,and the third generation of rejuvenated plants.The character starting occurrence when the rejuvenated plants has been cutting propagation to the fourth generation,which shows that the imported plants is the maternal seedling has been cutting propagation after three generations. |
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