Effect Of Low-dose-dopa On NKCC2,NCC And AQP2 In The Broiler Kidney

Dopamine is one of the most primary role of the catecholamine family.With the research of dopamine,it is proved that dopamine,functioning as a neurotransmitter,is strongly coupled with Tourette,schizophrenia,parkinsonism,etc.Low-dose dopamine has an ability of increasing sodium excretion and urine output,and inhibiting the reabsorption of water and sodium from kidney tubules,which is widely used in clinical medicine however not reported in broilers.NKCC2,NCC,AQP2 and other proteins can be regulated by kidney to keep the fluid balance of organism.In order to study how the low-dose dopamine effects NKCC2,NCC,and AQP2 of broilers,40 broilers were divided into two groups at random: control group(group C=20),dopamine group(group D=20).L-dopa was injected into the D group(5μg/kg/min),and the control group only received an injection of same amount of 0.9% saline.Histomorphology observation,biochemical analysis of blood and urine,western blot analysis and RT-q PCR analysis were used to detect all the targets.The results show that compared to the group C,after low-dose L-dopa received,the level of blood urine nitrogen(BUN),creatinine(Cre)and pulmonary artery pressure(m PAP)of group D has no significant difference(P>0.05).Though,with the long term L-dopa injection,protein levels of NKCC2,NCC,AQP2 were diminished significantly(P<0.05)in renal tubular epithelial cell membrane and these levels were increased in the cytoplasm(P<0.05).However the m RNA expression of NKCC2,NCC and AQP2 shows no obvious change(P>0.05).The research is mainly about the mechanism that low-dose L-dopa mediates the aquaporin and sodium channel protein of broilers from detecting the crucial protein of kidney water and sodium metabolic pathway.The finding suggests that low-dose L-dopa can cause continuous promotion of sodium excretion and urine output via shuttle from the cell membrane to cytoplasm.And the promotion does not effect the the glomerular filtration rate and blood pressure.