Domestication And Cultivation Characteristics Of Three Wild Medicinal Fungi

Phylloporia ribis,Phallus hadriani,Dityophora duplicata are wild edible and medicinal fungi.According to the study,the efficacy of P.ribis is similar with Lonicera japonica,which can be used for anti-inflammatory,detoxification,pain etc..At present,it has been made into health care products,and has excellent curative effect on various kinds of inflammation and cold.P.hadriani has cultivated a fruiting body in our laboratory,but not yet for large-scale cultivation.D.duplicata is a kind of excellent medicinal fungi with falling hematic fat,adjusting the action of fatty acid to prevent high blood pressure.Because of the limited amount of harvesting,the fungi has not been systematically studied,and their pharmacological studies also have been limited.This paper studied the isolation and identification of fungi,chemical composition content of mycelium and liquid fermentation.To prepare for large-scale industrial fermentation production.First of all,we collected the fruit body of wild P.ribis.Then,separation and identification of the fruit body,determine the tissue separated from mycelium is P.ribis.The study of solid solid culture method found the P.ribis mycelium could grow in wheat bran,malt juice,carrot,PDA,GPC,GPY solid culture medium.The growth rate was the fastest in the malt liquor solid medium.On the fiftieth day,the biomass of wheat bran solid medium was the highest.On the fortieth day,the content of the triperpenoid in the GPY medium was 0.39%.The highest of total triperpenoid was wheat bran culture medium for 60 days.The adaptability of P.ribis with carbon and organic nitrogen source is strong in the medium,good growth in carbon source with maltose,xylose,fructose and lactose.In addition to urea,it can grow in other nitrogen sources.P.ribis can grow in the liquid culture medium with carbon source,nitrogen source and growth factor.The best growth was in malt liquid culture medium which can be seed medium and fermentation medium.In shake flask fermentation,the content of triperpenoid was not less than 1%,and the content of polysaccharide was above 15%.The maximum biomass was in seventh days,while adenosine was the highest.Along with the prolonging of liquid fermentation time,the color of P.ribis mycelium become brown from white with the fermentation liquid color turned into brown,with a fresh fruit flavor.This study using tissue isolation method to isolate P.hadriani,the molecular biology identification shows that the mycelium isolated was P.hadriani.P.hadriani can grow in different solid medium,good growth in wheat bran,malt liquor,carrot and PDA solid medium.The adaptability of P.hadriani in carbon and organic nitrogen source is strong in the medium.The mycelial growth rate and the morphology of mycelia were different in different solid medium.Through the screening of different liquid medium showed that PDA liquid culture medium is suitable for the seed culture medium of P.hadriani.In the fermentation tank of 10 L,feeding7L,sucrose 140 g,soybean peptone 42 g,yeast extract 70 g,inoculation amount 5%,temperature of 26 DEG C,speed 150 rpm,ventilation volume 1:0.3,cultured to 58 h suitable for tank.The polysaccharide content of mycelium was 18.02%.The mycelium biomass and the sugar content in fermentation broth can be the standard for judging.D.duplicata can grow in different culture,good grown in GPY,PDA,malt juice,carrot,wheat bran solid medium.The growth rate was the fastest in GPY solid medium,and the highest biomass was 145.50 mg.The adaptability of D.duplicata in carbon and organic nitrogen source is very strong.D.duplicata liquid seed culture medium was PDA.In the fermentation tank of 10 L,feeding7L,sucrose 140 g,soybean peptone 42 g,yeast extract 70 g,inoculation amount 5%,temperature of 26 DEG C,speed 150 rpm,ventilation volume 1:0.2,cultured to 90 h suitable for tank.The polysaccharide content of mycelium was 17.92%.