Studies On The Gene Function Botrytis Cinerea By Coniothyrium Minitans Induce

Botrytis cinerea is a necrotrophic pathogenic fungus with worldwide distribution,and causes gray mold of the host plants.Although Botrytis cinerea has a closerelationship with Sclerotinia sclerotiorum,Coniothyrium minitans could parasitize S.sclerotiorum and hn454 strain wich like B.fabiopsis but not B.cinerea.In order to clarify the mechanisms underlying,differentially expressed genes BC1G₀0904,BC1G₁2653,BC1G₀3604 and BC1G₀0245 were screened from B.cinerea based on the RNA-seq results.In this resasearch,the function of these genes was studied through gene knockout and complementary technique.BC1G₀0904 encodes a putative protein containing a conserved domian of Mito-carr（Mitochondrial Carrier）,which is responsible for transporting the tricarboxylate substances.It catalyzes obligatory exchanges of the dibasic form of a tricarboxylic acid for another tricarboxylate/H+,a dicarboxylate or phosphoenolpyruvate in Saccharomyces cerevisiae.deletion of BC1G₀0904 resulted in decreases of virulence and conidial production as well as delay of sclerotial formation,while did not influence the growth and nohost parasitism.Production of ROS and H2O2 were also decreased.Complementary of BC1G₀0904 rescued all the abnormal characteristics.BC1G₁2653 and BC1G₀0245 encode conserved domian of MFS（Major Faciltator Superfamily）and GHS（Glyco_hydro₉2 superamily）,respectively,while BC1G₀3604 encodes a putative protein.BC1G₁2653 deletion mutants showed a lower virulence,growth rate and conodia production,and chould be parasitized by strain ZS-1 of C.minitans.BC1G₀3604 deletion mutants showed reduction in virulence,sclerotial production.BC1G₀0245 had no significant influence in biological characteristics of B.cinerea.The results obtained will help us to understand the mechanisms of the pathogenicity,conidiation and interaction with C.minitans on the molecular level.