| Botrytis cinerea is a notorious fungal pathogen with worldwide distribution,caused gray mold of the host plant. Sclerotia of B.cinerea canresist adverse environmental conditions and survive many years in soil. Under suitable environmental conditions, sclerotiacan germinated to conidia or mycelia to infectthe host plant. Gray mold deseaseis difficult to control in practice. Illustrating the mechanism of pathogencity and sclerotial development will be useful to the development of new fungicide and treatments. In this thesis, four genes（BcCVNH, BC1G09891, BC1G14375 and BC1G12206） with high-level expressi on during sclerotial development in RNA seq data were selected to study. BcCVNH contains a conserved domian of Cyano Virin-N Homology, BC1G09891 and BC1G14375 belong to Major Faciltator Superfamily, BC1G12206 belongs to a Hypothetical protein. A Real time RT-PCR results showed that gene BC1G05726express highly during infection stages and the others express highly during sclerotial development stage. These genes were knockouted according to the principle of homologous recombination and transformats were purified by single conidium isolation. BcCVNH deletion resulted in a reduction in virulence and conidia production, but the growth, sclerotia formation and hyphal tip morphology was not be affected. BC1G09891 deletion shows no significant effect on the growth, hyphal tip morphology and virulence of Botrytis cinerea, but showed faster sclerotia formationa,lesser conodia production and lower sclerotia germination rate. BC1G14375and BC1G12206 deletion show no significant effect on the sclerotia formation.Taken together, our research has demostrated the gene BcCVNH plays an important role in pathology and conidia production of Botrytia cinerea. The gene BC1G09891 deletion resulted in a reduction of conidia production, faster sclerotia formation and a lower sclerotia germination. The deletion of BC1G12206 and BC1G14375 have no obvious effect on sclerotia formation. |
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