Effects Of Dietary Sulfur-nitrogen Ratio On Ruminal Environmental Parameters, Gas Production And Nutrient Degradation Of Sheep In Artificial Simulation

In this experiment, the effects of four kinds of dietary nitrogen-sulfur ratio on rumen environment, microbial quantity and nutrient digestion were studied. By using the method in vitro simulation of different dietary nitrogen-sulfur ratio pH, volatile fatty acid (VFA), ammoniacal nitrogen (NH3-N), rumen bacterial crude protein (BCP) concentrated changes, the number of protozoa; the use of bag technology in different dietary nitrogen-sulfur ratio under the influence of rumen microbial metabolism on digestibility of nutrients. The effects of nutrient degradation rate influence, through a series of experiments in vitro fermentation of nitrogen-sulfur ratio on ruminant animal rumen microbes, reduce fermentation produced CH4 in order to find the most suitable nitrogen-sulfur ratio to meet the nutritional needs of ruminant animal, improve the utilization rate of feed, Total Mixed Rations (TMR) feed formulation reasonable to provide theoretical basis to provide a reasonable theoretical basis for the improvement of the environment.Test of healthy adult sheep rumen fluid, using a single factor design, set up four groups, with four kinds of nitrogen-sulfur ratio (6:1,7:1,8:1,9:1) of total mixed ration for experimental diets, each treatment consisted of four replications.The purpose of this study was to study the effects of four kinds of nitrogen-sulfur ratio diets on the internal environment of rumen fermentation. The test results showed that dietary nitrogen-sulfur ratio on rumen pH has remarkable effect, in a certain range, with the heighten of nitrogen-sulfur ratio, the pH of rumen tend to decrease; nitrogen-sulfur ratio is different: VFA, different amount of acetic acid, propionic acid and butyric acid concentration,7:1 group is the highest.9:1 is minimum. The concentration of acetic acid has a great influence on the concentration of other acids. The increase of dietary nitrogen-sulfur ratio, the concentration of rumen methane showed a downward trend, and the dietary nitrogen-sulfur ratio had a significant effect on the concentration of NH3-N and BCP in the rumen. The ratio in the rumen NH3-N and BCP concentration, the number of protozoa in different nitrogen-sulfur ratio, the most suitable is 8:1. The dietary nitrogen-sulfur ratio had significant effects on the cumulative gas production at different time points, and the trend of gas production in vitro was 9:1 group<8:1 group<7:1 group<6:1 group.In this study, the in vitro digestibility of different nitrogen-sulfur ratio was studied. Dietary nitrogen-sulfur ratio on CP and NDF at different time points of the cumulative degradation rate has the remarkable effect, in the fermentation time, the lower of the nitrogen-sulfur ratio’s value, the higher of the CP, NDF, ADF digestibility, CP degradation rate of nitrogen and sulfur with the highest ratio of 7:1, NDF and the highest degradation rate was 8:1. ADF and the highest degradation rate was 7:1 and 8:1.With the fermentation time of the extension of nitrogen and sulfur ratio, the ration of nitrogen and sulfur of between 7:1 and 8:1 of the nutrient utilization ratio is the best.