Stability And Stabilized Technologies Of Anthocyanins In Fruits Of Ligustrum Quihoui Carr.

In the present paper, the stability factors(temperature, p H value and metal ion) of anthocyanin extracts from Ligustrum lucidum fruit PH value were studied, and then the microencapsulation of anthocyanins extracts of Ligustrum lucidum were studied by means of piercing method and spray drying method, respectively. The microencapsulation process was optimized by investigating the embedding effect of anthocyanin extract of Ligustrum lucidum. The present research might lay a foundation for the further bioactivity research of anthocyanins extract of Ligustrum lucidum.First, the stability of anthocyanin from Ligustrum lucidum was studied. The results showed that at low temperature had little influence on the stability of anthocyanins of Ligustrum lucidum, however, when the temperature was higher than 75 ?, the degradation of anthocyanin was accelerated. The p H value had great influence on the stability of anthocyanins of Ligustrum lucidum. The preservation rate of anthocyanins was the highest at p H 4, and 91.77% at 4 h. When the p H value was higher than 4, the preservation rate of anthocyanin decreased rapidly. The effect of metal ions had a distinctive influence on the stability of anthocyanin in Ligustrum luciferum. The low concentration of Cu2+ reduced the stability of anthocyanin, and the concentration of Cu2+ higher than 10 mmol / L could improve the the stability of anthocyanins, Fe2+ had no effect on the stability of anthocyanins, while the stability of anthocyanins could be destroyed obviously by Fe3 +.The microcapsule preparation technology of anthocyanin from Ligustrum lucidum was studied by the piercing method. The preparation technology of anthocyanin microcapsule was optimized by single factor and orthogonal experiment. The experimental results were as follows. The single factor and orthogonal experiment showed that, the influence order of factors on the microencapsulation was as follow: wall material concentration > coagulant concentration > the ratio of core to wall, and the optimized microcapsulation parameters was that: wall material concentration 3%, coagulant concentration 2.5% and the ratio of core to wall 1: 2.5. After microencapsulation of anthocyanins, the content of anthocyanin in the microcapsule was 9.7%. In the simulated intestinal environment, only a small amount of anthocyanins was dissolved in microencapsulated anthocyanins after two hours. All of the anthocyanins were released completely after six hours, indicating that microcapsules anthocyanins have good controlled-release function.Then the spray drying method of anthocyanin microcapsule of Ligustrum lucidum was studied. Maltodextrin, acacia gum and ethyl cellulose were chosen as the wall material of single and double-layer microcapsule, ester and monoglyceride as the corresponding emulsifiers, soybean oil as the inner wall of the double-layer capsule, lecithin as the corresponding emulsifier. The anthocyanin microcapsule of Ligustrum lucidum was prepared according to single and double-layer anthocyanin microcapsule, respectively. The best technological parameters of microencapsulated emulsion were obtained respectively. The conditions of spray drying were determined and the products of single and double layer anthocyanin microcapsules were obtained. The results showed that the single-layer anthocyanin microcapsules had good dissolubility, transparent red color, high water content, high density and high embedding efficiency, which is suitable for the development and production of good color and embedding efficiency of anthocyanin encapsulated products. In comparison, double-layer anthocyanins microcapsules have a poor solubility, dissolved milk powder, low water content, low density, normal embedding efficiency, which is more applicable on the development and production of oil-soluble anthocyanin encapsulated products.The present study focuses on the researches of stability and stabilization of anthocyanins from Ligustrum lucidum. This paper could lay a foundation for the later study on the functional activity of Ligustrum lucidum product, as well as provide technical reference for the comprehensive utilization of Ligustrum lucidum plant.