| Radish (Raphanus sativus L.), belongs to the family of Cruciferae, is an annual or biennial herb. It is an important root vegetable in China. Radish is enriched in glucoraphenin(4-methylsulfiny-3-butenyl glucosinolate, RAE), which is an important member of glucosinolates (GLS). Sulforaphene (SFE), the hydrolysis product of glucoraphenin, has been proved to be one of the best anti-cancer activity components in vegetables so far. Studies have shown that sulforaphene plays an important role in inhibition of cell mutation and antioxidation. Nowadays many studies focus on the medical value of sulforaphene have been reported. However, there are few reports about determination of the content in different germplasms and exploration of the content change tendencies during growth, development and storage of radish.In this study, Uitrasonic-assisted extraction technology optimization of sulforaphene from radish (Raphanus Sativus L.) root and HPLC detection method. Fourty three and four germplasm were chosen, analysis of edibile and leaves, variation of sulforaphene content in different organs of radish during the development stages after germination, and research the nutritive losses, SFE concentration and mysosinase activity and texture analysis during storage at 4 ? for 4 months.The central results of this study were following:1. Uitrasonic-assisted extraction technology optimization of sulforaphene from radish(Raphanus Sativus L.) root. In this study, the ultrasonic-assisted extraction of sulforaphene from radish was optimized by using response surface methodology. According to the previous study of single variable test, an ultrasonic procedure for the extraction of sulroraphene from radish was established. A three-level-four-factor test was employed in this study to optimize the factorial parameters (ultrasonic power, ultrasonic time, water-bath time, water-bath temperature) by employing Box-Behnken design (BBD) of response surface methoodollogy,the content sulforaphene was taken as the response of the designed , and the regression equation is established: Y=390.26-35.56A-18.46B+15.77C-29.34D+26.31AB+1.37AC+8.88 AD-1.17BC+10.28BD-11.27CD-65.11AZ-39.45B2-46.62C2-142.31D2 . The fit indicators of the mathematical model by this study are acceptable. The results showed that the optimal extraction conditions for the extraction of sulforaphene from radish were ultrasonic time 20 min, ultrasonic power 40%, water-bath temperature 20 ? and 60 min.Under these conditions,the yield is close to the theoretical value.The response surface methodology is a workable method to optimize ultrasonic-extraction conditions of sulforaphene form radish.2. Analysis of SFE content in diverse radish gerpalsms. In this study, the contents of SFE were analyzed of 43 lines of radish roots. The differen between the content of SFE in radish roots were striking. The highest was about 10.8 times of the lowest, and the variety of hign SFE was less. Overall, the SFE content of red skin and white flesh radish is the highest,followed by green skin and red flesh radish, green skin and green flesh radish, white skin and white flesh radish. This information laid the foundation for mining the high SFE excellent genes and the radish quality genetic improvement.3. Four lines of radish (16Q1?16Q56?16Q62 and 16Q154),were chosen for study on variation of SFE conten in different organs of radish during different development stages. The study fistly revealed the different variances of SFE content in the development stages of radish, and there was higher content in root than in the leaves. The result provided the basis for the establishment of cultivation techniques of hign quality radish.4. Changes of quality indexes in Radish during Storage. In this study, Four lines of radish(16Q1?16Q56?16Q62 and 16Q154), were chosed for study on some quality indexes and texture characteristics during Storage for four months. At the beginning of storage, the content of vitamin C and the soluble sugar in the fleshy of radish were increased, the weight loss rate changed slowly, and the changes of SFE content and myrosinase activity were not obvious.With the storage duration, the weight loss rate of fleshy root increased gradually, and the contents of vitamin C, soluble sugar, SFE and the myrosinase activity were gradually decreased. The varieties 16Q1 and 16Q62 had lower variation of texture parameters during storage, and were storable character varieties. The 16Q56 and 16Q154 texture parameters of the cultivars decreased obviously, and the loss in content of moisture and nutrient of the fleshy tap roots were serious, which was not suitable for long-term storage of radish. |
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