Effects Of Growth Conditions On The Content Of Sulforaphene In Radish

Glucoraphenin(RAE)can be hydrolysed to sulforaphene(SFE)by myrosinase in radish.Sulforaphene plays an important role in preventing and fighting cancer because of its strong biological activity in anti-oxidant activity and anti-cellular mutation.The changes of SFE in fleshy roots and leaves of radish under different fertilization treatments,different seasons and different temperature and lighting conditions were studied by using the method of high performance liquid chromatography(HPLC).The main results were as follows:1.Fertilizers can affect the content of SFE in radish leaves and fleshy roots.Compared with organic fertilizers,chemical fertilizer was more conducive to the accumulation of SFE in the early stage of radish growth,while organic fertilizers was more conducive to the accumulation of SFE in the later stage of radish growth.In terms of different fertilization formulas,the treatment with the application amount of organic fertilizer not less than 2250kg/hm~2 had a significantly better effect than other treatments on increasing the content of SFE in mature stage.Among them,the application amount of organic fertilizer 2250 kg/hm~2,combined with the application of 225 kg/hm~2 nitrogen fertilizer and 225 kg/hm~2 compound fertilizer was the optimal treatment for improving the content of SFE in leaves and fleshy roots.Under the optimal fertilization formula,the contents of SFE in radish fleshy roots and leaves in spring cultivated radish reached 697.58?g/g DW and 441.02?g/g DW,which were76.08%and 21.40%higher than that of single chemical fertilizer,and the contents of SFE in single fertilizer treatment in autumn cultivated radish reached 552.88?g/g DW and 238.46?g/g DW,which were 17.26%and 21.15%higher than that of single chemical fertilizer.2.In the harvest period,the content of SFE cultivated in spring was higher than that in autumn,which was affected by environmental conditions such as temperature and light intensity.The content of SFE in different types of radish was affected to different degrees by cultural environmental conditions.The most significantly affected was the green-skinned and red-fleshed type radish named‘man tang hong'.The content of SFE in fleshy roots were1405.03 g/g DW and 371.87?g/g DW in the spring and autumn respectively,the content in spring was 277.83%higher than autumn.The least affected was the green-skinned and green-fleshed radish named‘weixian qing',whose content of SFE in fleshy roots was 496.16g/g DW and 430.49 g/g DW in spring and autumn respectively,the content of SFE in spring was 15.25%higher than autumn.The rapid accumulation stage of SFE in spring fleshy roots was 6-to 10-weeks after sowing,while in autumn 4-to 6-weeks after sowing.The content of SFE in radish leaves in spring was higher than in autumn cultivated radish in early and late growth period.The content of SFE in leaves and fleshy roots was most significant at the 4~thh weeks and the 12~thh week after sowing.3.The content of SFE in radish sprouts showed a gradual decrease with the growth time after budding.The law of its influence by environmental conditions was different from that of leaves and fleshy root.Growth temperature within the scope of the 22?~32?,low temperature was more conducive to the accumulation of SFE,the content of SFE in sprouts was higher with temperature varies from 22°C to 24°C.The daylight time was in the range of0~24 h,the content of SFE was the highest under the dark treatment.The sprouts growth well under the condition of 4~8 h in daylight,and the content of SFE was higher.Under different light quality conditions,the content of SFE in radish seedllings was the highest under dark treatment,yellow light treatment came second,however,content difference was not significant under red light,blue light and white light treatments.