Studies On The Tissue Culture Of Ormosia Henryi Prain

Ormosia henryi Prain is one kind of the second grade national key protective plants. As a valuable timber tree species and garden plant species, it’s fruit characteristic is existed for fema phenomenon, while the seed collection is very difficult. So establish Ormosia henryi Prain’s tissue culture system and expand reproduce coefficient is a effective way of rapid propagation. In this paper, in order to study the effect by different combination of reagent and time on the tissue culture, leaf and stem segment of seedling and adult tree has been chosen as explants, then sdutys of media’s selection, optimum culture condition’s control and substratum of transplanting in proliferation, robust nusery stock cultivation, rooting and seedling transplanting is developed. By two year’s research, the results are as fllows:1. The optimum explants collection time and combination of reagent of Ormosia henryi Prain on the tissue culture is finally-screened. The results showed that 70% alcohol soak 30 s +0.1%Hgcl soak 8 minutes +70% alcohol soak 30 s +PPM 0.5ml/L have the best sterilization effect for stem segment of ten days seedling. The optimum collecting time of seedling explants is April, the optimum sterilization method of leaf for seedling and stem segment for seedling are 70% alcohol soak 30 s +(50mg/L Rifampicin+50mg/L Chloramphennicol) +0.1%Hg Cl soak 6 minutes and 70% alcohol soak 30 s +(50mg/L Rifampicin+50mg/L Chloramphennicol) +0.1%Hg Cl soak 8 minutes. The optimum collecting time of leaf explants for adult tree’s is April, and the best sterilization method is 70% alcohol soak 30 s +(50mg/L Rifampicin+50mg/L Chloramphennicol) +0.1%Hg Cl soak 12 minutes, but the stem segment of adult tree has not achieved optimum sterilization effect.2. The technique system of rapid propagation of Ormosia henryi was established. The results showed the best fundamental medium for tissue culture of Ormosia henryi is MS, the rate was 80% and has less browning and vitrification phenomenon than WPM and B5, average bud numbers was 6.4; the most suitable medium for subculture was MS+6-BA2.0mg/L+NAA0.5mg/L+ agar 8g/L+ sucrose 30g/L, then 45 d later, the average number of buds was 6.4 buds; the suitable medium for rooting culture was 1/2WPM+IBA 1.0mg/L+NAA2.0mg/L+ agar 8g/L+ sucrose 10g/L,and the average root number was 5.9; tissue-cultured seedlings rooting well in different matrixes in course of out vitro rooting process, and the best temperature for rooting is 23℃.3. Solved the problems in soma clone domestication motheds of Ormosia henryi. Study on the motheds of soma clone domestication shows: the survival rate of tissue cultured seedling’s leaves was improved with the strengthen of overshadow rate, so only one layer of solar-shading screen can meet the needs of acclimatizition. Tissue cultured seedlings with 30 ds strong seedling cultivation growing well in four type of matrixes, the survival rate was 100%, but they grow better in the mixture of peat and vermiculite(1:1 in volume) and the mixture of peat, vermiculite and perlite(1:1:1 in volume); root nodule formed after about 30 d.