Study On The Application Of Oxytetracycline Residue As Dietary Protein Additive

Our country is abundant in the production and export country of antibiotic drug.In the process of antibiotics production, much oxytetracycline residue will be produced. If the residue is abandoned in the environment directly, it may result in environmental pollution and be a threat to man health and ecosystem due to the high moisture content, rich organic materials, and little residual titer. If the residual titer can be removed from the oxytetracycline residue, it allows to act as one kind of protein feed additives because of large amount of protein with a weight ratio of 40%, which will not only resolve the problem of pollution, but also turn the residue into valuable protein feed additives.This article aims to seek some effective strategies to separate the residual titer from the oxytetracycline residue. In the basis of the analysis about the protein content and oxytetracycline residue before and after coping with the oxytetracycline residue, residual titer can be removed by means of the chemical, ultrasonic and microwave ways. The experiment about the remove of residual titer from the oxytetracycline residue via chemical method proposed by Sun was performed, in order to make a comprehensive comparison between the advantages and shortcomings in the three ways mentioned above, and the nearly same conclusion was obtained. After the research into the optimal technique condition for the remove of residual titer with the three approaches, advantages and shortcomings of the three ways was achieved and the optimum schemes and technique condition to cope with oxytetracycline residue was acquired. At the same time, sub-chronic toxicity test was conducted by feeding the mouse with the coped oxytetracycline residue. At last, whether the coped residue was toxic or not was discussed.In the chapter three, several successive measurements including adding hydrochloric acid, adding methyl alcohol, using calcium oxide and heating were taken to reduce the residue titer in the a oxytetracycline residue to derive the best technique parameters. The detailed actions were as follows: firstly, 2.5g antibiotic drug was immersed in the 20.0mL hydrochloric acid solution with the concentration of 2.0mol/L for one hour; after this, the mixed solution was stirred for three hours under 95? water-bath and then was cooled to the room temperature; then the coped mixed solution was added with 10 m L methyl alcohol and stirred for one hour; after this step, the mixed solution was filtered; and then the filtered residue was mixed into 15 mL distilled water; after this the PH value of the residue solution was modulated to 5.0 with calcium oxide; and then the stirring step and filtering step was taken under the same condition as above-mentioned. In the end, the titer of residual titer was determined to be 605.3 U/g and the residue titer removed rate was 92.7%. The protein content of the coped antibiotic filtered residue was decided to be 38.3 % under check, 91.6% of which was retained.In the chapter four, the oxytetracycline residue was handled via ultrasonic wave and Fe0.Firstly, the Fe0 was directly added into the residue to explore the effect of Fe0 ratio on the residue titer; secondly, the residue was handled via antibiotic wave to determine the ratio of solid to liquid and the best operation time; lastly, the optimal condition to cope with residue was studied with the both ways. It turned out that when 0.1g Fe0 was added, the ratio of solid to liquid was 1:4 and the operation duration was 55 min, the residue titer was 1078.9U/g and the removal rate was 86.98%.In the chapter five, the residue was handled by microwave. We discussed the influence of temperature, ratio of solid to liquid and reaction duration on the removal titer respectively. On this basis, the orthogonal experiment was conducted to determine the best technique parameters for removing residue titer by microwave. It showed when the reaction duration was 40 min, the reaction temperature was 120? and the ratio of solid to liquid is 4:1, the residue titer was 552.6U/g and the removal rate was 93.33%. The protein content of the coped antibiotic filtered residue was decided to be 38.9% under check, 93.1% of which was retained.In conclusion, hydrochloric acid and methyl alcohol were used when dealing with the residue with chemical method, which may result in twice pollution and produce toxic materials due to the remaining chemical reagents. When theresidue was handled with ultrasonic wave and Fe0, we may face several problems, such as the low removal ratio of residue titer, much Fe in the drug, drying the residue and so on. So it is apparent that microwave method is the best way to cope with residue because no need of chemical reagents, satisfied experimental result and the good smell of residue.The Sun Group was invited to join the sub-chronic toxicity test which studying the effect of coped residue on the mouse. Judging from clinic symptom, no toxic materials were generated in removing residue with microwave method. At last, high-performance liquid chromatography was used to measure the content of oxytetracycline residue in kidney and liver of tested mice and it turned out that no antibiotic was observed. It demonstrated that the coped oxytetracycline residue can feed livestock and provide a reliable basis for it as dietary protein additive...