Effect Of BrcuHAC1 And BrcuPRMT5 Silencing By VIGS Induce On Cabbage Bolting And Flowering Time

Flowering Chinese Cabbage (Brassica rapa syn. Campestris L. ssp. Chinensis var. utiLis Tsen et Lee), also known as flowering, is our country cultivated in southern China's largest specialty vegetables,Brassicacamperstris is its product.Flower stalk formation is a kind of phenomenon of bolting, from flower bud differentiation to flower morphology built throughout the period of flower bud formation, accelerate the process of flower bud development, mature flower bud, bolting and flowering.This study use flowering Chinese cabbage premature early maturing variety varieties "Youqing 49" and late maturing variety "oil green sweet cabbage 80 days" as material, cloning the cabbage autonomous pathway genes BrcuHACl and BrcuPRMT5,and use virus inducing gene silencing (VIGS) technique to analyze the influence of these two genes in flowering time of cabbage bolting.Experimental methods and results are as follows:1.The conserved region sequence of AtHACl and AtPRMT5 in Arabidopsis thaliana as template primers were designed to obtain the full-length BrcuHACl and BrcuPRMT5 gene in flowering Chinese cabbage using RACE technology. The length of the two genes, the polymorphism of the exon of Chi Ko and exon, the comparison of homology and the function and structure of the protein were analyzed.2.Expression of gene BrcuHACl and BrcuPRMT5 in flowering Chinese cabbage of early and late maturing varieties of time is anlyasised by quantitative polymerase chain reaction (PCR).Experession of BrcuHACl and BrcuPRMT5 gene in Early maturing and late maturing varieties enhanced with the number of true leaves increased early maturing and late maturing varieties BrcuHACl and BrcuPRMT5 gene expression in floral organs in the strongest, stems and leaves in second, root undetectable expression of two genes.3.Further analysis of relationship between factor FLC BrcuHACl and BrcuPRMT5 gene and flowering inhibition by VIGS Technology.We selected two genes about 300bp fragment inserted into tobacco rattle virus vector TRY, infecting cabbage plants, after a period of time, the BrcuHACl gene, BrcuPRMT5 gene and the expression of the infected plants and control plants were analyzed.The results showed that the expression level of BrcuHACl and BrcuPRMT5 in infected plants was lower than that in control plants,but,the expression of BrcuFLC in infected plants was significantly higher than that in control plants.4. After a period of time, to observe the performance of transgenic and transgenic plants of early and late bolting significantly later than control plants.