The Relationship Between Genomic DNA Methylation And Catalpol Accumulation Of Rehmannia Glutinosa.F. Huechingensis

Rehmannia glutinosa belongs to Scrophulariaceae Rehmannia, is a kind of perennial herbaceous plants and belongs to one of the traditional Chinese medicinal materials. DNA methylation is one of the most common forms of epigenetic and plays an important role in regulating growth and development, genomic imprinting, stress response, and genne expression of plant. In this study,85-5 as a kind of experiment material was researched to find the differences of morphological structure, content of catalpol, level and patterns of DNA methylation under condition of different regions or concentrations of methylation inhibitors 5-azaC. Which would help to explore the correlation among place of origin, DNA methylation and Rehmannia morphology and catalpol accumulation of, then reveal the role of DNA methylation in the genuineness formation of Rehmannia, and also provide reference for the genuineness the mechanism of medicinal plants from the perspective of epigenetics. The main results were as follows:1?Different place of origin will be affect the phenotype of Rehmannia, the plant height of Wenxian Rehmannia was higher than that of Xinxiang, the leaf length of Wenxian Rehmannia is longer than that of Xinxiang; plant height of Rehmannia treated with 5-azaC was lower than CK, and with the concentration of 5-azaC increased, its inhibitory effect was more significant; 5-azaC make leaves of Rehmannia growth slowly, and with the concentration increase the inhibition is more significant.2?The catalpol content of Rehmannia root and leaf was determined using HPLC technology, and found that place of origin affect catalpol content of Rehmannia, the catalpol content of Wenxian Rehmannia root and leaf was more than that of Xinxiang; In different period, the catalpol content of Rehmannia root and leaf increased with the growth period, there will be a slight decrease after maturity. In addition, the catalpol content of Rehmannia root and leaf treated with 5-azaC were lower than control. In the root, in 15?iM to 100?M concentration range, catalpol content of treatment group was lower, with the higher concentration of 5-azaC. In the leaves, catalpol content in Rehmannia treated by different concentration of 5-azaC was lower than control at the same time, and along with the higher concentration the inhibitory effect was more significantly; The above results showe that 5-azaC has inhibitory effect on the accumulation of catalpol.3?Using HPLC technology to analyzes the genome methylation level of Rehmannia root and leaf, found that under different place of origin, there are differences in genome methylation level of Rehmannia. In Rehmannia genome treated with 5-azaC, the level of genome methylation was lower than the controls, gradually reduce and with the increase of 5-azaC concentration; In addition, there also have differences in level of genome methylation level to Rehmannia roots and leaves, which leaves methylation level was higher than the roots, genome methylation level of under the mature stage Rehmannia root and leaf was higher than the seedling stage.4?The methylation patterns of Rehmannia leaves and root was analyzed by MSAP technology, and found that Rehmannia was found to had tissue-specific of genome methylation status, there aslo have differences in of roots and leaves of the methylation degree; the degree and pattern of methylation in Rehmannia genome in different place of origin was different, the plants treated with 5-azaC were more in half methylation and no methylation status, which mean that the main change of methylation status was inside and outside at the genome GGCC site. In addition, with the increase 5-azaC concentration, the type and the number in genome demethylation of Rehmannia is increasing.5?The genome DNA methylation stripe of Rehmannia under the different place of origin was sequenced and analyszed, searched similar sequence through Blastx, and found the different DNA methylation would sequence be connected with retrotransposon protein, carbohydrate hydrolase, mitochondrial protein, chloroplast NADH, PPR protein and so on. M8 genes has a very important role in plant growth and development, the formation of organelles, RNA editing processing, and plays a crucial role in the transmission of signals between nucleus and organelles, was one of the largest family of proteins in plants and played a very important function in the normal growth and development of plants.