Effects Of Abamectin And Imidacloprid On Physiological And Biochemical Properties In Wheat Seedlings

Insecticides have been used for decades because of the economical and efficient advantage. The regular uses protect the crops from insects, increase the yield and encourage our agricultural production. Now the constant use made lots of negative effects on environment and the human health, which has aroused people's wide attention and realized the importance of comprehensive understanding and evaluation chemical pesticides. Though the disadvantages of chemical pesticides, we did ignore one thing that chemical pesticides also effect physiological and biochemical pesticides on crops. And the good news is that more and more scientists begin to study this.Today most of the studies about the effects of biochemical pesticides on crops did not suggest that the effects of different ingredients in pesticides, and in the meantime, we ignored the same named pesticides may have different formula. In this study, we selected two common pesticides, 2% Abamectin EC and 70% Imidacloprid WG, and the original medicines. We also chose two common pesticides additives, Glycol and Xylene. The novelty of this research is the analysis of the technical, commercial agents and additives on the different influence of wheat seedlings.We chose the same concentration as the agricultural production, diluted with acetone. Wheat seedling would be treated after getting 3 leaves. 24 h later we detected the following indicators: 1, changes of chloroplast pigment content, including the content of chlorophyll a, chlorophyll b, carotenoid content and total chlorophyll, the ratio of chlorophyll a and chlorophyll b; 2, concentrations of organic matter, such as soluble protein, soluble sugar, proline; 3, malondialdehyde content; 4, the activity of active oxygen enzyme, including SOD, POD, CAT, GPX and APX.These results show that:1, Effect of pesticides on chloroplast pigment.2% Abamectin EC and Xylene caused the chlorophyll a and chlorophyll b, carotenoid content decreased, but Abamectin Tech had little impact on them, which suggested that the pesticides containing Xylene additives had obvious poison on wheat seedlings, and the additives in Abamectin EC had the same damage to seedlings.2, Effect of pesticides on organic matter.Those six chemical substance dad an certain affect on content of organic matter, from which we can see that this chemicals is one environmental stress factor.3, Effect of pesticides on malondialdehyde.The content of MDA rose obviously after treated with 2% Abamectin EC and Xylene, which suggested that those two pesticides can make severe damage to wheat seedlings.4, Effect of pesticides on active oxygen enzyme.Abamectin EC dad a great effect on the activity of SOD?CAT and APX, while Xylene had a great effect on the activity of GPX, POD.We found that the six pesticides had an impact on physiology and biochemistry of wheat seedlings. Abamectin EC and Xylene were the most poisonous, imidacloprid and glycol were relatively safe, and abamectin tech had little impact on seedlings. We believe that the additives in pesticides formulation also have an obvious effect on crops. We should pay more attention to the additives not only the pesticides formulation.