Studies On Pharmacokinetics Of The Iconic Compounds Caffeine In Extract Of Green Tea In White Feather Broiler

With the development of Chinese national economy, the safety problems of the meat products has been paid more and more attention and healthy breeding was widely accepted. In this condition, it has become a new trend in the development of breeding industry to reduce the incidence of disease and drug residues by developing green pollution-free health care drugs. Tea is a kind of pure natural agricultural products with a variety of health care functions, and it's proven that if tea was added in the feed or drinking water could produce benefits both for the health care and disease prevention.In this experiment, white feather broilers were selected to analyze the pharmacokinetic process in vivo including absorption, distribution, metabolism and excretion to grasp the dynamically change in the body of white feather broilers. The aim of this study was provided a theoretical reference basis in broiler breeding of green tea extract as a kind of health care drug application.Blood and tissues were collected in accordance with pre-established schedule after orally administered 3 ml/kg body weight of tea extract(1ml is equivalent to 1g tea). Caffeine is rich in tea extract with the content is about 3.25%. Therefore, caffeine was elected as the flag compounds, utilizing HPLC, to make qualitative and quantitative analysis.Following oral dosing of the tea extract(1ml is equivalent to 1g tea), the values of the Cmax in plasma and tissues were 59.67±2.34(plasma), 20.41±0.29(heart), 11.26±0.27(liver),10.64±0.51(spleen), 11.37±0.75(lung), 7.35±0.11(kidney), 19.19±0.89(pectorales),19.80±0.24(crureus), 24.81±0.15(brain), 11.48±0.67(marrow), 5.53±0.47(reproductive organs) and 19.10±0.83(pancreas) ?g/m L, respectively. Meanwhile, the average elimination half-life(t1/2) in plasma and tissues were 12.95±0.49(plasma), 10.42±0.24(heart),9.94±0.19(liver), 10.04±0.21(spleen), 9.42±4.49(lung), 3.26±0.03(kidney), 7.23±0.02(pectorales), 11.00±0.46(crureus), 10.16±0.22(brain), 11.36±0.55(pancreas), 7.06±0.26(reproductive organs), 11.54±0.34(marrow) h, separately.The HPLC method for determination of caffeine in the plasma and tissues of the white feather broiler that established in this experiment met the requirements of the pharmacokinetic study. Following oral dosing of tea extract 4h later, the Cmax in plasma was attained, and linearity was confirmed in the concentration range 0.1–80 ?g/ml for caffeine in plasma.Following oral dosing of tea extract 4h later, the brain attained the highest Cmax in all the tissues, which confirmed that the caffeine could pass the brain-blood barrier. The eliminationrate constant in kidney was higher than that in plasma and other tissues, so this elucidated the caffeine was mainly excreted through kidney. The MRT of caffeine was pretty short, this meant there was no drug residue problem.