Molecular Identification And Inspection Of Important Pathogen And Insect Of Tobacco Plants

In China, tobacco is a kind of important economic crops, tobacco has been paid more and more concern by science researchers. Traditional methods of classification and recognition, we often identify on the basis of the tobacco which has occurred pests and diseases. So, the focus of this stage of the tobacco quality inspection is how to detect which tobacco has pests and diseases quickly and threats the quality of tobacco seriously. This passage is for studying and designing specificity primers to detect tobacco fungi containing alternaria alternate, colletotrichum nicotianae;insects including phestia elutell, lasioderma serricorne- A total of eight kinds of common pests and diseases on tobacco. By means of Molecular biology, we can identify the eight kinds of pests and diseases quickly and accurately. This can also provide a theoretical basis and technical guidance for tobacco import and export fast Inspection and quarantine technology.1. Discrimination detection A. alternate and C. nicotianaeCompared with colletotrichum nicotianae r DNA nucleotide sequence, we can simple design specificity primers for pcr amplification. As six different strains of anthrax DNA a template respectively, we can only specificity bands amplified from tobacco DNA in anthrax. As eight pathogenic fungus separating from tobacco DNA a template respectively, we can only specificity bands amplified from tobacco DNA in anthrax. Compared with alternaria alternata r DNA the sequence of nucleotides bases,we can design specificity primers for PCR amplification. As eight pathogenic fungus separating from tobacco DNA a template respectively, we can only specificity bands amplified from tobacco DNA in anthrax.2. Discern and test of P. syringae and R. solanacearumCompared with pseudomonas syringae rDNA nucleotide sequence, we can design specificity primers for PCR amplification. As pseudomonas syringae and ralstonia solanacearum DNA a template respectively, we can only specificity bands amplified from tobacco DNA in pseudomonas syringae. Compared with ralstonia solanacearum r DNA nucleotide sequence, we can calculate specificity primers for pcr amplification. As pseudomonas syringae and ralstonia solanacearum DNA a template respectively, we can only specificity bands amplified from tobacco DNA in pseudomonas syringae.3. Fast test TMVVirus(TMV) Compared with TMV cp gene nucleotide sequence, we can calculate specificity primers for pcr amplification. From fresh tobacco leaf, dried-up tobacco leaf diseased plants and cured tobacco leaves DNA, we can amplify the specificity bands.4. Molecular identification and detection of Phestia elutell and lasioderma serricorneAccording to the phestia elutell COI gene nucleotide sequence, calculate specificity primers for pcr amplification. Egg, larva, pupa, adult of phestia elutell-four kinds insect states are able to amplify bands.According to lasioderma serricorne COI gene nucleotide sequence, design specificity primers for PCR amplification. Egg,larva, pupa, adult of lasioderma serricorne-four kinds insect states are able to amplify bands.