The Study Of Relationship Between Extracellular Enzymes And Products Of Adenosine In Cordyceps

Cordyceps can infect insects form fruiting bodies produce adenosine,cordycepin and other biologically active substances.In order to solve the production of adenosine Cordyceps substrate appropriate amount of problems and avoid wastage of resources In this study,extracellular enzyme produced by Cordyceps,Cordyceps explore the relationship between extracellular enzyme adenosine with the product between the most prevalent.In addition,adenosine detection mainly by high performance liquid chromatography,high performance liquid chromatography costs,sample handling and instrument operation process cumbersome.If you can find the relationship between activity and product,we can solve nucleoside substance dependence on the HPLC method.Wort and potato juice culture Cordyceps militaris FFCC5142,detect extracellular enzyme activity and nucleosides during the cultivation.Potato juice can produce amylase,cellulase and pectinase,Wort can produce cellulase.Activity increased as the culture time first,then becomes low.The total amount of nucleoside rise slowly at first and then remained stable,Activity and nucleosides have no significant relationship.FFCC5142 cultured by wort culture and potato juice broth 10 d,then detection of adenosine and cordycepin by HPLC,generate4.17mg/L adenosine,3.25mg/L cordycepin and 0.66mg/L adenosine,0.77mg/L cordycepin.Further study the effects of carbon generated extracellular enzymes and products of FFCC5142.The carbon source is starch,pectin and CMC.The concentration factor and time factor experiments.2%,4% and 6% starch concentration,the time factor design three experiments,the culture period 20 d,sampling once every two days,colorimetry activity.Amylase activity at its 10 d,14d and 14 d enzyme activity reached maximum 39.72U/mL,35.68U/mL and 30.45U/mL.Nucleosides maximum reached 77.182 mg/L,291.984 mg/L and637.478 mg/L.At this point no adenosine.0.2% and 1% pectin concentration,the time factor experimental design two groups,0.2% and 1% pectin pectinase medium 4d and 8d at its maximum for the 6.68U/mL and 3.89U/m L.Accumulation of nucleoside reach 20mg/L and70mg/L.0.2% and 1% CMC concentration,two groups of the time factor experiment design,CMC enzyme activity reached a maximum value at 14 d 1.64U/mL and 1.35U/mL.Nucleosides yield are 35mg/L.Were combined with amylase,pectinase and CMC enzyme activity curve and nucleosides trends seen no significant relationship exists between the two.Starch,pectin and CMC broth without adenosine and cordycepin detected.Visible FFCC5142 natural deficient strain.In the medium were added four common growth factor adenine,guanine,hypoxanthine and adenosine.The results showed that starch medium were added hypoxanthine and adenine1g/L to produce adenosine were 3.6mg/L and 7.6mg/L.Pectin and CMC medium supplemented these four growth factor in not produce adenosine.