Study On Biological Characteristics And Some Active Component Of Cordyceps Gunnii

The biological characteristics and some active component of cordyceps gunnii was studied for the first time ,from growth survey and drawing of the spawn, the observation cordyceps gunnii and paecilomyces gunnii through scanning electron microscope , electrophoretic karyotype of cordyceps gunnii, to the contentation of active components ,such as cordyceps polysaccharide , amino acid , nucleoside in the spawn,tranma and worm of cordyceps gunnii .the result showed as below :Four typical growth periods in the spawn were studied : the period of growth hesitation, the rapid growth period ,the growth steady period,and the old period.There were plenty of cordyceps polysaccharide , amino acid , nucleoside in the spawn of cordyceps gunnii , their contentation in it was below : cordyceps polysaccharide,28.2mg/g ; amino acid ,28.62mg/100mg ; cytidine ,620.3 g/g; uridine , 3784.32 g/g; adenosine , 2588.17 g/g ; deoxyadenosine , 420.1 g/g . The total contentation of 7 kinds of nucleoside was 7402.4 u g/g .His and Pro were measured in amino acide of cordyceps gunnii for the first time.Electrophoretic karyotype of Cordyceps gunnii was studied for the first time .The spawn of C.gunnii was cultured with sabouraud culture medium,which was kept at 25 and vibrated at a speed of 120rpm for five days were best suitable for preparation of protoplasts .It was the optimum condition to prepare protoplasts that spawn was digested in 2% lywallzyme at 30 for 2 h with vibration at a speed of 120 rpm .The concentation of 4.3 108 /mL of protoplasts was the best suitable for electrophoresis . Cordyceps gunnii was separated into 6 bands ,which the biggest estimated to be 2100kb ,the smallest estimated to be 600kb.