| We constructed the hemocompatible hierarchical architecture on the thermoplastic elastomers based on polyoxyethylene-polyoxypropylene-polyoxyethylene tri-block polymer(Pluronic F127)micro/nano fibers through the reactive electrospinning.The F127-DA which had carbon-carbon double bonds in the macromolecular chains was prepared by acylation of F127.The crosslinking reactions among F127-DA and thermoplastic elastomers rendered the architecture stable.Based on the separation of F127-DA from other components in the electrospinning process,hydrophilic micro/nano structural fibers with F127-DA as the main component were prepared,which showed good blood compatibility.The content was provided in details:Part I: Preparation of blood compatible and anti-oxidative F127 micro/nano fibers.The micro/nano fibers with a stable crosslinked structure of F127-DA and Styrenebutadiene-Styrene(SEBS)block copolymers were prepared by reactive electrospinning technology with pentaerythritol tetrakis(3-mercaptopropionic acid)ester(PETM)as a crosslinker.Low protein adsorption,platelet adhesion,cell mechanical fragility,cytotoxicity and hemolytic rate demonstrated that the hydrophilic and anti-oxidative F127-DA/SIS/AA-2G fibers reduced the interactions between blood components and material surface,mechanical and oxidative damage of cells.In addition,red blood cells(RBCs)were preserved for 6 days in the bags of saturated styrene butadiene styrene block copolymer(SEBS),whose surfaces were covered with the electrospun fibers.The stored RBCs in these bags showed low hemolysis rate,cell mechanical fragility and oxidation degree,as well as normal shape of RBCs.Part II: Construction of oxidation-responsive and blood compatible surface on SEBS with F127-based micro/nano fibers.The micro/nano fibers of SEBS,F127-DA,copolymer of poly(ethylene glycol)diacrylate and 1,2-ethanedithiol(PEGDA-EDT)and antioxidants(AA-2G)were prepared by reactive electrospinning.Micro/nano fibers were characterized by surface morphology,responsiveness of reactive oxygen species(ROS),ROS-triggered release of AA-2G from microfibers,red blood cell storage in vitro,and blood compatibility.Experiments results showed the electrospun SEBS/F127-DA/PEGDA-EDT/AA-2G fibers not only possessed high blood compatibility,but also effectively controlled the release of AA-2G in the response to excess ROS,maintaining the normal physiological concentrations of ROS for normal cell function.Our work provided guidelines for the design of blood contact materials. |
